Western Blot (WB)
(Western Blot (WB) analysis of Caldesmon Rabbit mAb at 1:1000 dilutionLane1:NIH/3T3 whole cell lysate)
Rabbit Caldesmon Monoclonal Antibody | anti-CALD1 antibody
Caldesmon Rabbit Monoclonal Antibody
IHC: 1:50-1:200
ICC: 1:50-1:200
IF: 1:50-1:200
FC/FACS: 1:50-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of Caldesmon Rabbit mAb at 1:1000 dilutionLane1:NIH/3T3 whole cell lysate)
Western Blot (WB)
(Western Blot (WB) analysis of Caldesmon Rabbit mAb at 1:1000 dilutionLane1:NIH/3T3 whole cell lysate)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Caldesmon in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Caldesmon antibody. Counter stained with hematoxylin)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caldesmon antibody. Counter stained with hematoxylin.)
Immunocytochemistry (ICC)
(ICC staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Immunocytochemistry (ICC)
(ICC staining Caldesmon in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of NIH/3T3 cells with Caldesmon antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
NCBI and Uniprot Product Information
120-150kDa smooth muscle
NCBI Description
This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
Caldesmon: a cytoskeletal protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells. May act as a bridge between myosin and actin filaments. Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Five alternatively spliced isoforms have been described.
Protein type: Actin-binding
Chromosomal Location of Human Ortholog: 7q33
Cellular Component: cytoskeleton; myofibril; plasma membrane; actin cap; cytosol; actin cytoskeleton
Molecular Function: calmodulin binding; myosin binding; tropomyosin binding; actin binding
Biological Process: muscle contraction; cell motility
Research Articles on CALD1
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Product Notes
The CALD1 cald1 (Catalog #AAA3014132) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Caldesmon Rabbit Monoclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Caldesmon can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 IF: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the CALD1 cald1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Caldesmon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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