Rabbit anti-Human CA14  Monoclonal Antibody | anti-CA14  antibody

Rabbit anti Human CA14  Monoclonal Antibody

Cat. Num. AAA2543998

• Gene Names: CA14; CAXiV
• Reactivity: Human
• Applications: ELISA
• Synonyms: CA14 ; Monoclonal Antibody; Rabbit anti Human CA14 Monoclonal Antibody; anti-CA14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 337

• Applicable Applications for anti-CA14 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human CA14. The detection limit for Human CA14 is approximately 0.0195 ng/well.
• Immunogen: Recombinant Human CA14 protein
• Buffer: This antibody was obtained from a rabbit immunized with purified, recombinant Human CA14/Car14 extracellular domain (rh CA14; NP_036245.1; Met 1-Met 290).
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -70 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-CA14  antibody

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29-46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.

NCBI and Uniprot Product Information

• NCBI GI #: 6912284
• NCBI GeneID: 23632
• NCBI GenBank Nucleotide #: NM_012113.2
• Molecular Weight: 37,668 Da
• NCBI Official Full Name: carbonic anhydrase 14
• NCBI Official Synonym Full Names: carbonic anhydrase 14
• NCBI Official Symbol: CA14
• NCBI Official Synonym Symbols: CAXiV
• NCBI Protein Information: carbonic anhydrase 14
• UniProt Protein Name: Carbonic anhydrase 14
• UniProt Gene Name: CA14
• UniProt Synonym Gene Names: CA-XIV
• UniProt Entry Name: CAH14_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. [provided by RefSeq, Jul 2008]

Uniprot Description

CA14: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family.

Protein type: EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: plasma membrane

Molecular Function: carbonate dehydratase activity

Biological Process: bicarbonate transport