Mouse anti-Human CA125 Monoclonal Antibody | anti-CA125 antibody
MOUSE ANTI HUMAN CA125
Gene Names
MUC16; CA125
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Synonyms
CA125; Monoclonal Antibody; MOUSE ANTI HUMAN CA125; anti-CA125 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
X325
Form/Format
Purified IgG - liquid
Concentration
Approx. Protein Concentrations: IgG concentration 1.0mg/ml (varies by lot)
Applicable Applications for anti-CA125 antibody
ELISA (EIA), Immunohistology - Paraffin, Western Blot (WB)
Application Notes
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Target Species
Human
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
TRIS buffered saline
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Immunogen
CA125 antigen purified from human ovarian carcinoma.
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Histology Positive Control Tissue
Lung adenocarcinoma, serosal ovarian adenocarcinoma and urinary bladder adenocarcinoma tissue.
Preparation and Storage
Store at +4°C or at -20°C if preferred.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody.
Related Product Information for anti-CA125 antibody
Mouse anti Human CA125 antibody, clone X325 recognizes the ovarian cancer mucin CA125 (also known as MUCIN 16). CA125 consists of a short cytoplasmic tail, a transmembrane domain and an exceptionally large glycosylated extracellular domain. The extracellular domain is dominated by a large number of 156-amino acid repeat units, over 60 in all. These repeats are known to bind the antibodies OC125 and M-11. CA125 is present within normal ovarian, endometrium, endocervix and fallopian tissue, but levels are elevated in over 90% of women with advanced ovarian cancer. CA125 levels may also be elevated in other cancers and non-cancerous conditions such as peritonitis, endometriosis and liver cirrhosis. Levels may also fluctuate during the menstrual cycle and occasionally increases in pregnancy, making CA125 an unreliable marker for ovarian cancer in premenopausal women. The function of CA125 in healthy tissue is unknown but it is thought to provide a protective lubricating barrier against particles and infectious agents at mucosal surfaces.
CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X325 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X325 has been successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the detection of CA125 antigen in human plasma samples.
CA125 was concluded by the International Society of Oncology and BioMarkers (ISOBM) to contain two major antigenic domains. Mouse anti Human CA125 antibody, clone X325 recognizes the epitope specificity group B, similar to the M-11 antibody (Nustad et al. 2002). Mouse anti Human CA125 antibody, clone X325 has been successfully as both a capture and detection reagent in the development of sensitive ELISA assays for the detection of CA125 antigen in human plasma samples.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
mucin-16
NCBI Official Synonym Full Names
mucin 16, cell surface associated
NCBI Official Symbol
MUC16
NCBI Official Synonym Symbols
CA125
NCBI Protein Information
mucin-16; CA125 ovarian cancer antigen; ovarian cancer-related tumor marker CA125; ovarian carcinoma antigen CA125
UniProt Protein Name
Mucin-16
UniProt Gene Name
MUC16
UniProt Synonym Gene Names
CA125; MUC-16; CA-125
UniProt Entry Name
MUC16_HUMAN
Uniprot Description
MUC16: Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Protein type: Membrane protein, integral; Membrane protein, peripheral; Cell adhesion
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: extracellular space; extrinsic to membrane; Golgi lumen; integral to membrane; plasma membrane; vesicle
Molecular Function: protein binding
Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; O-glycan processing; post-translational protein modification; cell adhesion
Research Articles on CA125
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Product Notes
The CA125 muc16 (Catalog #AAA214596) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CA125 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CA125 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistology - Paraffin, Western Blot (WB). Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CA125 muc16 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CA125, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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