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Rat anti-C4b
C4d C4 Monoclonal Antibody | anti-C4 antibody

C4, Mouse, mAb 16D2

Gene Names
C4b; C4; Ss
Reactivity
C4b
C4d
Applications
Immunofluorescence, Immunoprecipitation, Western Blot
Synonyms
C4; Monoclonal Antibody; Mouse; mAb 16D2; Complement component 4; anti-C4 antibody
Ordering
For Research Use Only!
Host
Rat
Reactivity
C4b
C4d
Clonality
Monoclonal
Isotype
Rat IgG2a
Form/Format
1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Sequence Length
44
Applicable Applications for anti-C4 antibody
Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Application Notes: W: A non-reduced sample treatment and SDS-Page was used. The band size is 200x kDa (Ref.4).
IA: Plates were coated overnight with rat anti-murine C4 mAb 16D2 in carbonate buffer and blocked with 5% dry milk in PBS and 0.01% Tween 20.
IF: Spleens were snap frozen and stored at -80 degree C until cryosections were cute. (Section (5um) were fixed for 4 mins in ice-cold acetone. Sections were blocked with 2% BSA, 2% FCS and PBS. Biotinylated 16D2 was visualized using avidin-PE.
Application Use: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Immunogen
Thymocytes decorated with Thy-1 antibody and complement components
Positive Control
T-cell regions of mouse spleen
Preparation and Storage
Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
Related Product Information for anti-C4 antibody
Product Description: The monoclonal antibody 16D2 recognizes mouse complement factor C4, formerly known as Gg protein, which consists of an alpha-, beta-, and gamma-chain. The classical pathway of complement and the Mannose binding lectin activation pathway converge at C4. C1s, MASP-1 and MASP-2 cleave C4 resulting in the formation of C4a and C4b. Subsequently, C4b can be cleaved to C4c and C4d by other serum enzymes. The monoclonal antibody 16D2 reacts with intact C4, C4b and C4d.
C4 is an acute phase protein that is produced by hepatocytes, monocytes and intestinal epithelial cells and can be used- in experimental animals as a marker for activation of the classical complement pathway. Recent studies have demonstrated an association between graft rejection and C4d deposition in a mouse model for cardiac transplantation.
References
1. Kremmer, E et al; Monoclonal antibodies to complement components without the need of their prior purification. II. Antibodies to mouse C3 and C4. Hybridoma 1990, 9 : 309 2. Gadjeva, M et al; Macrophage-derived complement component C4 can restore humoral immunity in C4-deficient mice. J Immunol 2002, 169: 5489
3. Paul E et al; Anti-DNA autoreactivity in C4-deficient mice. Eur J Immunol 2002, 32: 2672
4. Murata, K et al; Synergistic deposition of C4d by complement-activating and non-activating antibodies in cardiac transplants. Am J Transplant 2007, 7: 2605

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
192,915 Da
NCBI Official Full Name
C4, partial
NCBI Official Synonym Full Names
complement component 4B (Chido blood group)
NCBI Official Symbol
C4b
NCBI Official Synonym Symbols
C4; Ss
NCBI Protein Information
complement C4-B; complement component 4 (within H-2S); complement component 4B (Childo blood group)
UniProt Protein Name
Complement C4-B
Protein Family
UniProt Gene Name
C4b
UniProt Synonym Gene Names
C4
UniProt Entry Name
CO4B_MOUSE

Uniprot Description

C4B: C4 plays a central role in the activation of the classical pathway of the complement system. It is processed by activated C1 which removes from the alpha chain the C4a anaphylatoxin. The remaining alpha chain fragment C4b is the major activation product and is an essential subunit of the C3 convertase (C4b2a) and the C5 convertase (C3bC4b2a) enzymes of the classical complement pathway. Defects in C4B are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Interindividual copy- number variation (CNV) of complement component C4 and associated polymorphisms result in different susceptibilities to SLE. The risk of SLE susceptibility has been shown to be significantly increased among subjects with only two copies of total C4. A high copy number is a protective factor against SLE. Defects in C4B are the cause of complement component 4B deficiency (C4BD). A rare defect of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus with or without associated glomerulonephritis.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: complement binding; complement component C1q binding; carbohydrate binding

Biological Process: immunoglobulin mediated immune response; complement activation

Research Articles on C4

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Product Notes

The C4 c4b (Catalog #AAA584166) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The C4, Mouse, mAb 16D2 reacts with C4b C4d and may cross-react with other species as described in the data sheet. AAA Biotech's C4 can be used in a range of immunoassay formats including, but not limited to, Immunoassay (IA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB). Application Notes: W: A non-reduced sample treatment and SDS-Page was used. The band size is 200x kDa (Ref.4). IA: Plates were coated overnight with rat anti-murine C4 mAb 16D2 in carbonate buffer and blocked with 5% dry milk in PBS and 0.01% Tween 20. IF: Spleens were snap frozen and stored at -80 degree C until cryosections were cute. (Section (5um) were fixed for 4 mins in ice-cold acetone. Sections were blocked with 2% BSA, 2% FCS and PBS. Biotinylated 16D2 was visualized using avidin-PE. Application Use: For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the C4 c4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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