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Mouse anti-Chicken c-Kit Monoclonal Antibody | anti-KIT antibody

Mouse Anti-Chicken c-Kit-PE

Gene Names
KIT; PBT; SCFR; C-Kit; CD117
Reactivity
Chicken
Applications
Flow Cytometry
Synonyms
c-Kit; Monoclonal Antibody; Mouse Anti-Chicken c-Kit-PE; Mouse Anti-Chicken c-Kit - Phycoerythrin-Labeled; Mouse Anti-Chicken c-Kit; anti-KIT antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
Mouse (BALB/c) IgG2a kappa
Clone Number
kit2c75
Specificity
Chicken c-Kit
c-Kit is a 145 kDa transmembrane receptor-type protein tyrosine kinase encoded by the c-Kit protooncogene and is the receptor for stem cell factor (SCF). 1 c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. 2, 3, 4, 5 The amino acid sequence of chicken c-kit shows 63% identity to mouse c-Kit and exhibits similar expression patterns in RNA blot analysis suggesting similar biological roles.1
Form/Format
R - phycoerythrin (R - PE) Conjugate
Sequence Length
22
Applicable Applications for anti-KIT antibody
Flow Cytometry
Application Notes
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Immunogen
Sp2/0 cells, Sp/chkit4A4, transfected with chicken c-kit cDNA1
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to characteristics of a standard reference reagent.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Sasaki, E., H. Okamura, T. Chikamune, Y. Kanai, M. Watanabe, M. Naito, and M. Sakurai. 1993. Gene. 128:257.
2. Yee, N.S., I. Paek, and P. Besmer. 1994. J. Exp. Med. 179:1994.
3. Iemura, A., M. Tsai, A. Ando, B.K. Wershil, and S.J. Galli. 1994. Am. J. Pathol. 144:321.
4. Blume-Jensen, P., L. Claesson-Welsh, A. Siegbahn, K.M. Zsebo, B. Westermark, and C.H. Heldin. 1991. EMBO J. 10:4121.
5. Kinashi, T. and T.A. Springer. 1994. Blood 83:1033.
6. Vainio, O., D. Dunon, F. Aissi, J.P. Dangy, K.M. McNagny, and B.A. Imhof. 1996. J. Cell Biol. 135:1655.
7. Lampisuo, M., K. Katevuo, and O. Lassila. 1998. Scand. J. Immunol. 48:52.
8. Lampisuo, M. J. Liippo, O. Vainio, K.M. McNagny, J. Kulmala, and O. Lassila. 1999. Int. Immunol. 11:63.
9. Schulte, C.E., M. von Lindern, P. Steinlein, H. Beug, and L.M. Wiedemann. 2002. EMBO J. 21:4297.
10. Katevuo, K., B.A. Imhof, R. Boyd, A. Chidgey, A. Bean, D. Dunon, T.W. Gobel, and O. Vainio. 1999. J. Immunol. 162:5685.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
109,865 Da
NCBI Official Full Name
c-Kit
NCBI Official Synonym Full Names
v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog
NCBI Official Symbol
KIT
NCBI Official Synonym Symbols
PBT; SCFR; C-Kit; CD117
NCBI Protein Information
mast/stem cell growth factor receptor; OTTHUMP00000158901; OTTHUMP00000218587; proto-oncogene c-Kit; soluble KIT variant 1; tyrosine-protein kinase Kit; proto-oncogene tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein
UniProt Protein Name
Mast/stem cell growth factor receptor
UniProt Gene Name
KIT
UniProt Entry Name
KIT_HUMAN

NCBI Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

Function: This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).

Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure: Interacts with APS. Interacts with MPDZ (via the tenth PDZ domain). Interacts with PTPRU. Ref.7 Ref.8 Ref.9

Subcellular location: Membrane; Single-pass type I membrane protein.

Involvement in disease: Defects in KIT are a cause of piebald trait (PBT) [

MIM:172800]. PBT is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. Ref.13 Ref.14 Ref.15 Ref.17 Ref.18 Ref.19 Ref.20 Ref.22 Ref.27Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [

MIM:606764]. Ref.19 Ref.23 Ref.24 Ref.28 Ref.29Defects in KIT have been associated with testicular tumors (TEST) [

MIM:273300]. A common solid malignancy in males. Germ cell tumors of the testis constitute 95% of all testicular neoplasms. Ref.19

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 5 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 protein kinase domain.

Research Articles on KIT

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Product Notes

The KIT kit (Catalog #AAA673955) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Chicken c-Kit-PE reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's c-Kit can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry. Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the KIT kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Kit, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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