Human laminin, alpha 5 ELISA Kit | LAMA5 elisa kit

Human Laminin-5, LN-5 ELISA Kit

Cat. Num. AAA703569

• Reactivity: Human
• Synonyms: laminin; alpha 5; Human Laminin-5; LN-5 ELISA Kit; Human Laminin-5 (LN-5) ELISA Kit; RP11-157P1.6; KIAA1907; laminin alpha 5; laminin alpha-5 chain; LAMA5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human LAMA5. No significant cross-reactivity or interference between Human LAMA5 and analogues was observed.
• Sequence Length: 3695

• Detection Range: 12.5 ng/ml-800 ng/ml
• Sensitivity: 3.12 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Cell Adhesion
• Protein Biological Process 3: Cell adhesion
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LAMA5 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LN-5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for LN-5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain LN-5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of LN-5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21264602
• NCBI GeneID: 3911
• NCBI Accession #: NP_005551.3
• NCBI GenBank Nucleotide #: NM_005560.4
• UniProt Accession #: O15230; Q8TDF8; Q8WZA7; Q9H1P1
• Molecular Weight: 399,737 Da
• NCBI Official Full Name: laminin subunit alpha-5
• NCBI Official Synonym Full Names: laminin, alpha 5
• NCBI Official Symbol: LAMA5
• NCBI Protein Information: laminin subunit alpha-5; laminin alpha-5 chain; laminin-10 subunit alpha; laminin-11 subunit alpha; laminin-15 subunit alpha
• UniProt Protein Name: Laminin subunit alpha-5
• Protein Family: Laminin
• UniProt Gene Name: LAMA5
• UniProt Synonym Gene Names: KIAA0533; KIAA1907
• UniProt Entry Name: LAMA5_HUMAN

NCBI Description

This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]

Uniprot Description

Function: Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.