Immunofluorescence/Immunocytochemistry (IF/ICC)
Mouse Bub1 Monoclonal Antibody | anti-Bub1 antibody
Mouse Anti-Bub1 [14H5]
Reactivity
Mouse, Human. Predicted to work with: Rat, Xenopus laevis, Kangaroo
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Western Blot, Immunohistochemistry
Purity
Protein A purified
Synonyms
Bub1; Monoclonal Antibody; Mouse Anti-Bub1 [14H5]; BUB1 budding uninhibited by benzimidazoles 1 homolog antibody; BUB1 budding uninhibited by benzimidazoles 1homolog (yeast) antibody; BUB1A antibody; BUB1L antibody; Budding uninhibited by benzimidazoles 1 (yeast homolog)antibody; Budding uninhibited by benzimidazoles 1 homolog antibody; hBUB1 antibody; Mitotic checkpointserine/threonine protein kinase BUB1 antibody; Mitotic spindle checkpoint kinase antibody; Putativeserine/threonine protein kinase antibody; anti-Bub1 antibody
Host
Mouse
Reactivity
Mouse, Human. Predicted to work with: Rat, Xenopus laevis, Kangaroo
Clonality
Monoclonal
Isotype
IgG1
Clone Number
14H5
Purity/Purification
Protein A purified
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Concentration
1.1mg/ml (varies by lot)
Applicable Applications for anti-Bub1 antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try 1g/ml final concentration
Target Antigen
Bub1
Immunogen
Fusion protein, corresponding to amino acids 281-419 of Human Bub 1
Myeloma/fusion Partners
SP2/0
Research Areas
Cell Division; Spindle
Immunofluorescence/Immunocytochemistry (IF/ICC)
Immunofluorescence/Immunocytochemistry (IF/ICC)
References
Phosphorylation and PoloBoxdependent Binding of Plk1 to Bub1 Is Required for the Kinetochore Localization of Plk1 Wei Qi et al (MBC Online,August 2006) ICC/IF(Human)PMID: 16760428 Boyarchuk Y et al. Bub1 is essential for assembly of the functional inner centromere. J Cell Biol 176:919-28 (2007)PMID: 17389228 Kittler R et al. Genome-scale RNAi profiling of cell division in human tissue culture cells. Nat Cell Biol. 2007 Dec;9(12):1401-12. Epub 2007 Nov 11. ICC/IF(Human)PMID: 17994010 Stavropoulou V et al. TPPII promotes genetic instability by allowing the escape from apoptosis of cells with activated mitotic checkpoints. Biochem Biophys Res Commun. 2006 Jul 28;346(2):415-25. Epub 2006 Jun 2. WB(Human)PMID: 16762321
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Product Notes
The Bub1 (Catalog #AAA375059) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Bub1 [14H5] reacts with Mouse, Human. Predicted to work with: Rat, Xenopus laevis, Kangaroo and may cross-react with other species as described in the data sheet. AAA Biotech's Bub1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Optimal antibody dilution should be determined by titration, however as a guideline try 1g/ml final concentration. Researchers should empirically determine the suitability of the Bub1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bub1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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