Rabbit anti-Human BTK Monoclonal Antibody | anti-BTK antibody

BTK (Phospho-Y223) Rabbit Monoclonal Antibody

Cat. Num. AAA3014164

• Gene Names: BTK; AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A affinity purified
• Synonyms: BTK; Monoclonal Antibody; BTK (Phospho-Y223) Rabbit Monoclonal Antibody; Tyrosine-protein kinase BTK; Agammaglobulinemia tyrosine kinase; ATK; B-cell progenitor kinase; BPK; Bruton tyrosine kinase; AGMX1; anti-BTK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Specificity: This antibody detects endogenous levels of BTK protein only when phosphorylated at Tyr223.
• Purity/Purification: Protein A affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 659

• Applicable Applications for anti-BTK antibody: Western Blot (WB)
• Application Notes: WB: 1:1000-1:2000
• Immunogen: Recombinant antibody.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of BTK (phospho-Y223) Rabbit mAb at 1:1000 dilutionLane1:K562 whole cell lysate treated with pervanadateLane2:K562 whole cell lysate)

Related Product Information for anti-BTK antibody

Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases. Like other BTK family members, it contains a pleckstrin homology (PH) domain, Src homology SH3 and SH2 domains. BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3, 4, 5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of BTK is negatively regulated by PKC beta through phosphorylation of BTK at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation.

NCBI and Uniprot Product Information

• NCBI GI #: 4557377
• NCBI GeneID: 695
• NCBI Accession #: NP_000052.1
• NCBI GenBank Nucleotide #: NP_000052.1
• UniProt Accession #: Q06187
• Molecular Weight: ~ 77kDa
• NCBI Official Full Name: tyrosine-protein kinase BTK isoform 1
• NCBI Official Synonym Full Names: Bruton tyrosine kinase
• NCBI Official Symbol: BTK
• NCBI Official Synonym Symbols: AT; ATK; BPK; XLA; IMD1; AGMX1; IGHD3; PSCTK1
• NCBI Protein Information: tyrosine-protein kinase BTK
• UniProt Protein Name: Tyrosine-protein kinase BTK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: BTK
• UniProt Synonym Gene Names: AGMX1; ATK; BPK; ATK; BPK
• UniProt Entry Name: BTK_HUMAN

NCBI Description

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]

Uniprot Description

Btk: a tyrosine kinase of the Tec family. Plays a crucial role in B-cell ontogeny. Defects cause X-linked agammaglobulinemia, an immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. Truncated splice forms found in childhood leukemias may underlie radiation resistance of tumors through inhibition of apoptosis.

Protein type: Protein kinase, TK; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); Kinase, protein; TK group; Tec family

Chromosomal Location of Human Ortholog: Xq21.33-q22

Cellular Component: extrinsic to internal side of plasma membrane; mast cell granule; intracellular membrane-bound organelle; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; lipid raft

Molecular Function: identical protein binding; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; metal ion binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; adaptive immune response; transcription, DNA-dependent; B cell activation; cell maturation; calcium-mediated signaling; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cell proliferation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; regulation of B cell cytokine production; MyD88-dependent toll-like receptor signaling pathway; regulation of B cell apoptosis; B cell receptor signaling pathway; histamine secretion by mast cell; toll-like receptor signaling pathway; innate immune response; positive regulation of B cell differentiation; mesoderm development; cell differentiation; toll-like receptor 4 signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Isolated Growth Hormone Deficiency, Type Iii; Agammaglobulinemia, X-linked