Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Mouse BrdU-incorporated Kidney stained with BrdU Rabbit Recombinant Monoclonal Antibody (BRD2888R).)
Rabbit anti-All species Bromodeoxyuridine (BrdU) Monoclonal Antibody | anti-BrdU antibody
Bromodeoxyuridine (BrdU) (Proliferation Marker)
Reactivity
All species
Applications
Immunohistochemistry
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Synonyms
Bromodeoxyuridine (BrdU); Monoclonal Antibody; Bromodeoxyuridine (BrdU) (Proliferation Marker); Bromodeoxyuridine; BUdr; anti-BrdU antibody
Host
Rabbit
Reactivity
All species
Clonality
Monoclonal
Isotype
IgG
Clone Number
BRD2888R
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-BrdU antibody
Immunohistology (Formalin-Fixed)
Application Notes
Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 minutes at RT; For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37 degree C.
Immunogen
Bromodeoxyuridine (BrdU) conjugated to KLH
Positive Control
Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU
Cellular Localization
Nuclear
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Mouse BrdU-incorporated Kidney stained with BrdU Rabbit Recombinant Monoclonal Antibody (BRD2888R).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Mouse BrdU-incorporated Kidney stained with BrdU Rabbit Recombinant Monoclonal Antibody (BRD2888R).)
SDS-Page
(SDS-PAGE Analysis Purified BrdU Rabbit Recombinant Monoclonal Antibody (BRD2888R). Confirmation of Purity and Integrity of Antibody.)
SDS-Page
(SDS-PAGE Analysis Purified BrdU Rabbit Recombinant Monoclonal Antibody (BRD2888R). Confirmation of Purity and Integrity of Antibody.)
Related Product Information for anti-BrdU antibody
Recombinant Rabbit Monoclonal Antibody
Similar Products
Product Notes
The BrdU (Catalog #AAA4380754) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Bromodeoxyuridine (BrdU) (Proliferation Marker) reacts with All species and may cross-react with other species as described in the data sheet. AAA Biotech's Bromodeoxyuridine (BrdU) can be used in a range of immunoassay formats including, but not limited to, Immunohistology (Formalin-Fixed). Immunohistology (Formalin-Fixed): 0.5-1ug/ml for 30 minutes at RT; For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37 degree C. Researchers should empirically determine the suitability of the BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bromodeoxyuridine (BrdU), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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