Mouse anti-Human BRF1 Monoclonal Antibody | anti-BRF1 antibody

Mouse monoclonal antibody Anti-Human BRF1

Cat. Num. AAA120050

• Gene Names: BRF1; BRF; CFDS; hBRF; BRF-1; GTF3B; TAF3C; TAF3B2; TF3B90; TAFIII90; TFIIIB90; HEL-S-76p
• Reactivity: Human
• Applications: Dot Blot, Immunocytochemistry
• Synonyms: BRF1; Monoclonal Antibody; Mouse monoclonal antibody Anti-Human BRF1; BRF1 homolog; subunit of RNA polymerase III? transcription initiation factor IIIB (S. cerevisiae) (BRF1); BRF; hBRF; GTF3B; TAF3C; TAF3B2; TF3B90;TAFIII90; TFIIIB90; MGC105048; anti-BRF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: BRF1G2A8
• Concentration: 100 ug/ml (1.0 ml) (varies by lot)
• Sequence Length: 3677

• Applicable Applications for anti-BRF1 antibody: Dot Blot (DB), Immunocytochemistry (ICC)
• Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum.
• Sterility: Filtered through a 0.22 um membrane.

Quality Control

(Western blot analysis of immunized recombinant protein, using anti-BRF1 monoclonal antibody. )

Quality Control #2

(Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Immunocytochemistry (ICC)

(Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRF1 mAb. [Lot No. BRF1G2A8-2] )

Related Product Information for anti-BRF1 antibody

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Three alternatively spliced variants encoding three different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. A transcript encoding a fourth isoform has not yet been completely characterized. [NCBI Entrez Gene Summary]

NCBI and Uniprot Product Information

• NCBI GI #: 338753403
• NCBI GeneID: 2972
• NCBI Accession #: NP_001510.2
• NCBI GenBank Nucleotide #: NM_001519
• Molecular Weight: 47,978 Da
• NCBI Official Full Name: Homo sapiens BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit (BRF1), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
• NCBI Official Symbol: BRF1
• NCBI Official Synonym Symbols: BRF; CFDS; hBRF; BRF-1; GTF3B; TAF3C; TAF3B2; TF3B90; TAFIII90; TFIIIB90; HEL-S-76p
• NCBI Protein Information: transcription factor IIIB 90 kDa subunit; B - related factor 1; BRF1 homolog, subunit of RNA polymerase III transcription initiation factor IIIB; TATA box binding protein (TBP)-associated factor 3C; TATA box binding protein (TBP)-associated factor, RNA polymerase III, GTF3B subunit 2; TBP - associated factor, RNA polymerase III, 90kD; epididymis secretory sperm binding protein Li 76p; general transcription factor IIIB, 90kD subunit
• UniProt Protein Name: Transcription factor IIIB 90 kDa subunit
• Protein Family: Transcription factor
• UniProt Gene Name: BRF1
• UniProt Entry Name: TF3B_HUMAN

NCBI Description

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]

Uniprot Description

GTF3B: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. Belongs to the TFIIB family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 14q

Cellular Component: nucleoplasm; transcription factor TFIIIB complex

Molecular Function: zinc ion binding; TATA-binding protein binding

Biological Process: transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; tRNA transcription; regulation of transcription, DNA-dependent; gene expression; regulation of mRNA stability; rRNA transcription

Disease: Cerebellofaciodental Syndrome