Western Blot (WB)
(BRF1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BRF1 antibody detected the BRF1 protein (arrow).)
Rabbit anti-Human BRF1 Polyclonal Antibody | anti-BRF1 antibody
BRF1, ID (BRF1, BRF, GTF3B, TAF3B2, TAF3C, Transcription factor IIIB 90kD subunit, B-related factor 1, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Western Blot (WB)
(BRF1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BRF1 antibody detected the BRF1 protein (arrow).)
Western Blot (WB)
(BRF1 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the BRF1 antibody detected the BRF1 protein (arrow).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]
Uniprot Description
GTF3B: General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter. Belongs to the TFIIB family. 7 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription initiation complex
Chromosomal Location of Human Ortholog: 14q
Cellular Component: nucleoplasm; transcription factor TFIIIB complex
Molecular Function: zinc ion binding; TATA-binding protein binding
Biological Process: transcription from RNA polymerase III promoter; transcription initiation from RNA polymerase III promoter; tRNA transcription; regulation of transcription, DNA-dependent; gene expression; regulation of mRNA stability; rRNA transcription
Disease: Cerebellofaciodental Syndrome
Research Articles on BRF1
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Product Notes
The BRF1 brf1 (Catalog #AAA6004454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The BRF1, ID (BRF1, BRF, GTF3B, TAF3B2, TAF3C, Transcription factor IIIB 90kD subunit, B-related factor 1, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BRF1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the BRF1 brf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BRF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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