Mouse anti-Human Brain Natriuretic Peptide (BNP) Monoclonal Antibody | anti-BNP antibody

Monoclonal Antibody to Brain Natriuretic Peptide (BNP)

Cat. Num. AAA2085854

• Gene Names: NPPB; BNP
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Protein A + Protein G affinity chromatography
• Synonyms: Brain Natriuretic Peptide (BNP); Monoclonal Antibody; Monoclonal Antibody to Brain Natriuretic Peptide (BNP); GC-B; NPPB; B-Type Natriuretic Peptide; Natriuretic Peptide Precursor B; Gamma-brain natriuretic peptide; Brain natriuretic peptide 32; anti-BNP antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b Kappa
• Clone Number: C3
• Specificity: The antibody is a mouse monoclonal antibody raised against BNP. It has been selected for its ability to recognize BNP in immunohistochemical staining and western blotting.
• Purity/Purification: Protein A + Protein G affinity chromatography
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-BNP antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Application Notes: Western Blot: 0.5-5ug/ml; Immunocytochemistry in formalin fixed cells: 5-30ug/ml; Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml; Immunohistochemistry in paraffin section: 5-30ug/ml; Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
• Immunogen: OVA Conjugated Brain Natriuretic Peptide (BNP)
• Cross Reactivity: Human, Pig
• Buffer Formulation: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
• Label: None
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Porcine Skeletal muscle lysate; Primary Ab: 2ug/ml Mouse Anti-Human BNP Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Stomach Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Brain Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Human Lung Cancer Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 4505433
• NCBI GeneID: 4879
• NCBI Accession #: NP_002512.1
• NCBI GenBank Nucleotide #: NM_002521.2
• UniProt Accession #: P16860; Q6FGY0; Q9P2Q7; B0ZBE9
• Molecular Weight: 14,726 Da
• NCBI Official Full Name: natriuretic peptides B preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Uniprot Description

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.22

Cellular Component: extracellular region; extracellular space; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: diuretic hormone activity; hormone activity; peptide hormone receptor binding; protein binding; receptor binding

Biological Process: body fluid secretion; cell surface receptor signaling pathway; cGMP biosynthetic process; inflammatory response; negative regulation of angiogenesis; negative regulation of cell growth; negative regulation of smooth muscle cell proliferation; negative regulation of systemic arterial blood pressure; protein folding; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of vascular permeability; response to calcium ion; response to drug; response to hypoxia; response to lipopolysaccharide; response to organic cyclic compound; response to peptide hormone