Mouse anti-Human Brachyury Monoclonal Antibody | anti-TBX1 antibody

Brachyury (Brachyury Homolog, Brachyury Protein, Bry, MGC104817, T, T Protein, TFT)

Cat. Num. AAA606573

• Gene Names: TBX1; DGS; TGA; CAFS; CTHM; DGCR; DORV; VCFS; TBX1C
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Brachyury; Monoclonal Antibody; Brachyury (Brachyury Homolog; Brachyury Protein; Bry; MGC104817; T; T Protein; TFT); Anti -Brachyury (Brachyury Homolog; anti-TBX1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: 9H202
• Specificity: Recognizes human brachyury.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide.

• Applicable Applications for anti-TBX1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic human brachyury peptide, aa224-435. Epitope: C-terminus.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TBX1 antibody

The Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.

Product Categories/Family for anti-TBX1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 2735863
• NCBI GeneID: 6899
• UniProt Accession #: Q152R5; O43435
• Molecular Weight: 40,353 Da
• NCBI Official Full Name: brachyury
• NCBI Official Synonym Full Names: T-box 1
• NCBI Official Symbol: TBX1
• NCBI Official Synonym Symbols: DGS; TGA; CAFS; CTHM; DGCR; DORV; VCFS; TBX1C
• NCBI Protein Information: T-box transcription factor TBX1; brachyury; OTTHUMP00000197322; OTTHUMP00000197323; OTTHUMP00000197324; Testis-specific T-box protein; T-box 1 transcription factor C
• UniProt Protein Name: T-box 1
• Protein Family: Brachyury protein
• UniProt Gene Name: TBX1
• UniProt Entry Name: Q152R5_HUMAN

NCBI Description

This gene is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. This gene product shares 98% amino acid sequence identity with the mouse ortholog. DiGeorge syndrome (DGS)/velocardiofacial syndrome (VCFS), a common congenital disorder characterized by neural-crest-related developmental defects, has been associated with deletions of chromosome 22q11.2, where this gene has been mapped. Studies using mouse models of DiGeorge syndrome suggest a major role for this gene in the molecular etiology of DGS/VCFS. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]

Uniprot Description

TBX1: Probable transcriptional regulator involved in developmental processes. Is required for normal development of the pharyngeal arch arteries. Haploinsufficiency of the TBX1 gene is responsible for most of the physical malformations present in DiGeorge syndrome (DGS) and velocardiofacial syndrome (VCFS). DGS is characterized by the association of several malformations: hypoplastic thymus and parathyroid glands, congenital conotruncal cardiopathy, and a subtle but characteristic facial dysmorphology. VCFS is marked by the association of congenital conotruncal heart defects, cleft palate or velar insufficiency, facial dysmorpholgy and learning difficulties. It is now accepted that these two syndromes represent two forms of clinical expression of the same entity manifesting at different stages of life. Defects in TBX1 are a cause of DiGeorge syndrome (DGS). Defects in TBX1 are a cause of velocardiofacial syndrome (VCFS). Defects in TBX1 are a cause of conotruncal heart malformations (CTHM). CTHM consist of cardiac outflow tract defects, such as tetralogy of Fallot, pulmonary atresia, double-outlet right ventricle, truncus arteriosus communis, and aortic arch anomalies. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: nucleus

Molecular Function: protein dimerization activity; protein homodimerization activity; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: heart morphogenesis; retinoic acid receptor signaling pathway; muscle development; heart development; positive regulation of transcription, DNA-dependent; muscle cell fate commitment; cell fate specification; middle ear morphogenesis; ear morphogenesis; anterior/posterior pattern formation; sensory perception of sound; epithelial cell differentiation; positive regulation of MAPKKK cascade; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; thyroid gland development; mesoderm development; angiogenesis; neural crest cell migration; muscle morphogenesis; blood vessel development; tongue morphogenesis; inner ear morphogenesis; pharyngeal system development; transcription, DNA-dependent; thymus development; outer ear morphogenesis; semicircular canal morphogenesis; embryonic viscerocranium morphogenesis; social behavior; embryonic cranial skeleton morphogenesis; pattern specification process; parathyroid gland development; odontogenesis of dentine-containing teeth; regulation of transcription from RNA polymerase II promoter; cell proliferation; negative regulation of cell differentiation; artery morphogenesis; blood vessel morphogenesis; blood vessel remodeling; positive regulation of transcription from RNA polymerase II promoter; soft palate development; positive regulation of protein amino acid phosphorylation; lymph vessel development; determination of left/right symmetry; vagus nerve morphogenesis; positive regulation of epithelial cell proliferation

Disease: Conotruncal Heart Malformations; Digeorge Syndrome; Velocardiofacial Syndrome; Tetralogy Of Fallot