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Western Blot (WB) (Western Blot analysis of various cells using LPAAT-? Polyclonal Antibody diluted at 1?500)

Rabbit LPAAT-delta Polyclonal Antibody | anti-LPAAT-delta antibody

LPAAT-delta Polyclonal Antibody

Gene Names
AGPAT4; 1-AGPAT4; dJ473J16.2; LPAAT-delta; RP3-473J16.2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
LPAAT-delta; Polyclonal Antibody; LPAAT-delta Polyclonal Antibody; AGPAT4; 1-acyl-sn-glycerol-3-phosphate acyltransferase delta; 1-acylglycerol-3-phosphate O-acyltransferase 4; 1-AGP acyltransferase 4; 1-AGPAT 4; Lysophosphatidic acid acyltransferase delta; anti-LPAAT-delta antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
LPAAT-delta Polyclonal Antibody detects endogenous levels of LPAAT-delta protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Applicable Applications for anti-LPAAT-delta antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1/500-1/2000
ELISA: 1/40,000
Immunogen
The antiserum was produced against synthesized peptide derived from human AGPAT4. AA range: 151-200
Preparation and Storage
Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of various cells using LPAAT-? Polyclonal Antibody diluted at 1?500)

Western Blot (WB) (Western Blot analysis of various cells using LPAAT-? Polyclonal Antibody diluted at 1?500)

Western Blot (WB)

(Western blot analysis of lysates from HeLa and MCF-7 cells, using AGPAT4 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from HeLa and MCF-7 cells, using AGPAT4 Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of the lysates from HeLa cells using AGPAT4 antibody.)

Western Blot (WB) (Western blot analysis of the lysates from HeLa cells using AGPAT4 antibody.)
Related Product Information for anti-LPAAT-delta antibody
Background: 1-acylglycerol-3-phosphate O-acyltransferase 4(AGPAT4) Homo sapiens. This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. [provided by RefSeq, Jul 2008]

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
44,021 Da
NCBI Official Full Name
1-acyl-sn-glycerol-3-phosphate acyltransferase delta
NCBI Official Synonym Full Names
1-acylglycerol-3-phosphate O-acyltransferase 4
NCBI Official Symbol
AGPAT4
NCBI Official Synonym Symbols
1-AGPAT4; dJ473J16.2; LPAAT-delta; RP3-473J16.2
NCBI Protein Information
1-acyl-sn-glycerol-3-phosphate acyltransferase delta; 1-AGPAT 4; 1-AGP acyltransferase 4; lysophosphatidic acid acyltransferase delta; lysophosphatidic acid acyltransferase-delta (LPAAT-delta); 1-acylglycerol-3-phosphate O-acyltransferase 4 (lysophosphati
UniProt Protein Name
1-acyl-sn-glycerol-3-phosphate acyltransferase delta
UniProt Gene Name
AGPAT4
UniProt Synonym Gene Names
1-AGP acyltransferase 4; 1-AGPAT 4; LPAAT-delta
UniProt Entry Name
PLCD_HUMAN

NCBI Description

This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. [provided by RefSeq, Jul 2008]

Uniprot Description

AGPAT4: Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Protein type: Lipid Metabolism - glycerolipid; Membrane protein, multi-pass; Transferase; Membrane protein, integral; EC 2.3.1.51; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: 1-acylglycerol-3-phosphate O-acyltransferase activity

Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; triacylglycerol biosynthetic process; cellular lipid metabolic process; CDP-diacylglycerol biosynthetic process; phospholipid biosynthetic process

Research Articles on LPAAT-delta

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Product Notes

The LPAAT-delta agpat4 (Catalog #AAA3002592) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LPAAT-delta Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LPAAT-delta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1/500-1/2000 ELISA: 1/40,000. Researchers should empirically determine the suitability of the LPAAT-delta agpat4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LPAAT-delta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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