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Western Blot (WB) (The Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain tissue lysate.)

Mouse anti-Human BMP7 Monoclonal Antibody | anti-BMP7 antibody

BMP7 antibody

Gene Names
BMP7; OP-1
Reactivity
Human
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
By protein-G affinity chromatography
Synonyms
BMP7; Monoclonal Antibody; BMP7 antibody; Bone morphogenetic protein 7; Osteogenic protein1; OP-1; Bone morphogenetic protein 7 BMP 7; Eptotermin alfa; OP 1; OP1.; anti-BMP7 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
4E7
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-BMP7 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000.
Immunogen
Recombinant human BMP7 (293-431aa) purified from E. coli
Preparation and Storage
Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain tissue lysate.)

Western Blot (WB) (The Mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP7 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Mouse Brain tissue lysate.)

Western Blot (WB)

(The extracts of mouse kidney (MK) and rat heart (RH) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP7 antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Western Blot (WB) (The extracts of mouse kidney (MK) and rat heart (RH) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BMP7 antibody (1:1,000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Immunocytochemistry/Immunofluorescence (ICC/IF)

(ICC/IF analysis of BMP7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BMP7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). )

Immunocytochemistry/Immunofluorescence (ICC/IF) (ICC/IF analysis of BMP7 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human BMP7 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green). )
Related Product Information for anti-BMP7 antibody
BMP-7 (bone morphogenetic protein-7), also known as OP-1 (osteogenic protein-1), is a member of the transforming growth factorbeta (TGFbeta) superfamily. BMP-7 plays an essential role during ontogeny of the mammalian eye and kidney, also, is expressed in heart, lung, small intestine, limb bud and teeth. In addition, noggin, one of the soluble BMP antagonists, regulated the action of BMPs on mesenchymal precursor cells, partially through a feedback type of inhibition.
Product Categories/Family for anti-BMP7 antibody
References
Gregory KE., et al. (2005) J. Biol, Chem. 280, 27970-27980.; Sampath Tk., et al. (1990) J. Biol. Chem. 265, 13198-13205; Godin RE., et al. (1998) Development. 125, 3473-3482; Groppe J., et al. (2002) Nature. 420, 636-642

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
655
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
bone morphogenetic protein 7 preproprotein
NCBI Official Synonym Full Names
bone morphogenetic protein 7
NCBI Official Symbol
BMP7
NCBI Official Synonym Symbols
OP-1
NCBI Protein Information
bone morphogenetic protein 7
UniProt Protein Name
Bone morphogenetic protein 7
UniProt Gene Name
BMP7
UniProt Synonym Gene Names
OP1; BMP-7; OP-1

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone, kidney and brown adipose tissue development. Additionally, this protein induces ectopic bone formation and may promote fracture healing in human patients. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Research Articles on BMP7

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Product Notes

The BMP7 bmp7 (Catalog #AAA200028) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BMP7 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BMP7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000. Researchers should empirically determine the suitability of the BMP7 bmp7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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