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Western Blot (WB) (Western blot analysis of BMP2K over-expressed 293 cell line, cotransfected with BMP2K Validated Chimera RNAi (Cat # H0MBS60522619-R01V) (Lane 2) or non-transfected control (Lane 1). Blot probed with BMP2K monoclonal antibody (M03) clone X1. GAPDH (36.1 kDa) used as specificity and loading control.)

Mouse BMP2K Monoclonal Antibody | anti-BMP2K antibody

BMP2K (BMP2 Inducible Kinase, BIKE, DKFZp434K0614, DKFZp434P0116, HRIHFB2017) (FITC)

Gene Names
BMP2K; BIKE; HRIHFB2017
Applications
Immunofluorescence, Western Blot
Purity
Purified
Synonyms
BMP2K; Monoclonal Antibody; BMP2K (BMP2 Inducible Kinase; BIKE; DKFZp434K0614; DKFZp434P0116; HRIHFB2017) (FITC); BMP2 Inducible Kinase; HRIHFB2017; anti-BMP2K antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a,k
Clone Number
X1
Specificity
Recognizes BMP2K.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Sequence Length
662
Applicable Applications for anti-BMP2K antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
BMP2K (AAH36021, 540aa-650aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
YQQAFFQQQMLAQHQPSQQQASPEYLTSPQEFSPALVSYTSSLPAQVGTIMDSSYSANRSVADKEAIANFTNQKNISNPPDMSGWNPFGEDNFSKLTEEELLDREFDLLRS
Conjugate
FITC
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of BMP2K over-expressed 293 cell line, cotransfected with BMP2K Validated Chimera RNAi (Cat # H0MBS60522619-R01V) (Lane 2) or non-transfected control (Lane 1). Blot probed with BMP2K monoclonal antibody (M03) clone X1. GAPDH (36.1 kDa) used as specificity and loading control.)

Western Blot (WB) (Western blot analysis of BMP2K over-expressed 293 cell line, cotransfected with BMP2K Validated Chimera RNAi (Cat # H0MBS60522619-R01V) (Lane 2) or non-transfected control (Lane 1). Blot probed with BMP2K monoclonal antibody (M03) clone X1. GAPDH (36.1 kDa) used as specificity and loading control.)

Testing Data

(Detection limit for recombinant GST tagged BMP2K is approximately 0.1ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged BMP2K is approximately 0.1ng/ml as a capture antibody.)

Western Blot (WB)

(Western Blot analysis of BMP2K expression in transfected 293T cell line by BMP2K monoclonal antibody (M03), clone X1.Lane 1: BMP2K transfected lysate (73.9 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of BMP2K expression in transfected 293T cell line by BMP2K monoclonal antibody (M03), clone X1.Lane 1: BMP2K transfected lysate (73.9 KDa).Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to BMP2K on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to BMP2K on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to BMP2K on HeLa cell. [antibody concentration 10 ug/ml])

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to BMP2K on HeLa cell. [antibody concentration 10 ug/ml])
Related Product Information for anti-BMP2K antibody
This gene is the human homolog of mouse BMP-2-inducible kinase. Bone morphogenic proteins (BMPs) play a key role in skeletal development and patterning. Expression of the mouse gene is increased during BMP-2 induced differentiation and the gene product is a putative serine/threonine protein kinase containing a nuclear localization signal. Therefore, the protein encoded by this human homolog is thought to be a protein kinase with a putative regulatory role in attenuating the program of osteoblast differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Product Categories/Family for anti-BMP2K antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
BMP2 inducible kinase
NCBI Official Synonym Full Names
BMP2 inducible kinase
NCBI Official Symbol
BMP2K
NCBI Official Synonym Symbols
BIKE; HRIHFB2017
NCBI Protein Information
BMP-2-inducible protein kinase

NCBI Description

This gene is the human homolog of mouse BMP-2-inducible kinase. Bone morphogenic proteins (BMPs) play a key role in skeletal development and patterning. Expression of the mouse gene is increased during BMP-2 induced differentiation and the gene product is a putative serine/threonine protein kinase containing a nuclear localization signal. Therefore, the protein encoded by this human homolog is thought to be a protein kinase with a putative regulatory role in attenuating the program of osteoblast differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Research Articles on BMP2K

Similar Products

Product Notes

The BMP2K (Catalog #AAA6178563) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BMP2K can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BMP2K for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BMP2K, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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