Western Blot (WB)
(Western blot analysis of 20 ng of HIV-1 p24 protein with PM-6585-biotin at 0.2 μg/mL.)
Mouse anti-Virus HIV-1 p24 Monoclonal Antibody | anti-GAG antibody
HIV-1 p24 Antibody [7F4]
Reactivity
Virus
Applications
ELISA, Western Blot
Purity
HIV-1 p24 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Synonyms
HIV-1 p24; Monoclonal Antibody; HIV-1 p24 Antibody [7F4]; gag Pr55(Gag); anti-GAG antibody
Host
Mouse
Reactivity
Virus
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7F4
Specificity
By Western blot, anti-HIV-1 p24 antibody detects a ~24 kDa, a ~41 kDa, and a ~55 kDa protein, corresponding to HIV-1 p24 and to its precursors p41 and p55, respectively, in HIV-1 samples.
Purity/Purification
HIV-1 p24 Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
500
Applicable Applications for anti-GAG antibody
ELISA (EIA), Western Blot (WB)
Application Notes
PM-6585-biotin can be used for detection of p24 by Western blot or ELISA at 0.2 - 0.5mug/mL.
Conjugate
Biotin
Immunogen
Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
Buffer
HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
HIV-1 p24 Monoclonal Antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year.
Western Blot (WB)
(Western blot analysis of 20 ng of HIV-1 p24 protein with PM-6585-biotin at 0.2 μg/mL.)
Western Blot (WB)
(Western blot analysis of 20 ng of HIV-1 p24 protein with PM-6585-biotin at 0.2 μg/mL.)
Related Product Information for anti-GAG antibody
HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 has been used as an integral part of multicomponent HIV-1 vaccines (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Predicted: 24; 41; 55 kDa
NCBI Official Full Name
gag polyprotein
NCBI Official Symbol
gag
NCBI Protein Information
Pr55(Gag); matrix; capsid; nucleocapsid; p2; p6; p1
UniProt Protein Name
Gag polyprotein
Protein Family
UniProt Gene Name
gag
UniProt Synonym Gene Names
MA; CA; NC
UniProt Entry Name
GAG_HV1H2
Uniprot Description
Gag polyprotein: Mediates, with Gag-Pol polyrotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi).
Research Articles on GAG
Similar Products
Product Notes
The GAG gag (Catalog #AAA153324) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HIV-1 p24 Antibody [7F4] reacts with Virus and may cross-react with other species as described in the data sheet. AAA Biotech's HIV-1 p24 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). PM-6585-biotin can be used for detection of p24 by Western blot or ELISA at 0.2 - 0.5mug/mL. Researchers should empirically determine the suitability of the GAG gag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HIV-1 p24, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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