Human Olfactory receptor 10A2 (OR10A2) ELISA Kit | OR10A2 elisa kit

Human Olfactory receptor 10A2 (OR10A2) ELISA Kit

Cat. Num. AAA756369

• Gene Names: OR10A2; OST363; OR10A2P; OR11-82; OR11-86
• Reactivity: Human
• Synonyms: Olfactory receptor 10A2 (OR10A2); Human Olfactory receptor 10A2 (OR10A2) ELISA Kit; OR10A2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This 2, 5-OAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 2, 5-OAS. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This 2, 5-OAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 2, 5-OAS. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OR10A2 elisa kit

Principle of the Assay: 2, 5-OAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-2, 5-OAS antibody and an 2, 5-OAS-HRP conjugate. The assay sample and buffer are incubated together with 2, 5-OAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 2, 5-OAS concentration since 2, 5-OAS from samples and 2, 5-OAS-HRP conjugate compete for the anti-2, 5-OAS antibody binding site. Since the number of sites is limited, as more sites are occupied by 2, 5-OAS from the sample, fewer sites are left to bind 2, 5-OAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 2, 5-OAS concentration in each sample is interpolated from this standard curve.

Product Categories/Family for OR10A2 elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 52317178
• NCBI GeneID: 341276
• NCBI Accession #: NP_001004460.1
• NCBI GenBank Nucleotide #: NM_001004460.1
• Molecular Weight: 33,817 Da
• NCBI Official Full Name: olfactory receptor 10A2
• NCBI Official Synonym Full Names: olfactory receptor, family 10, subfamily A, member 2
• NCBI Official Symbol: OR10A2
• NCBI Official Synonym Symbols: OST363; OR10A2P; OR11-82; OR11-86
• NCBI Protein Information: olfactory receptor 10A2; hP4 olfactory receptor; olfactory receptor OR11-86; olfactory receptor OR11-82 pseudogene; olfactory receptor, family 10, subfamily A, member 2 pseudogene
• UniProt Protein Name: Olfactory receptor 10A2
• Protein Family: Olfactory receptor
• UniProt Gene Name: OR10A2
• UniProt Synonym Gene Names: OR10A2P
• UniProt Entry Name: O10A2_HUMAN

NCBI Description

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Uniprot Description

OR10A2: Odorant receptor (Potential). Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1; Receptor, GPCR

Chromosomal Location of Human Ortholog: 11p15.4

Cellular Component: integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; olfactory receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; detection of chemical stimulus involved in sensory perception of smell