Immunohistochemistry (IHC)
(Formalin-paraffin tonsil stained with biotinylated Lambda Light Chain probe followed by Biotin MAb (SPM375). Note cell membrane & cytoplasmic staining.)
Mouse Biotin Monoclonal Antibody
Biotin (Vitamin B7, Vitamin H) (FITC)
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
Biotin; Monoclonal Antibody; Biotin (Vitamin B7; Vitamin H) (FITC); Vitamin B7; Vitamin H; anti-Biotin antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
SPM375
Specificity
Recognizes Biotin. Species Crossreactivity: all species.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-Biotin antibody
Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
FC/FACS: 0.5-1ug/million cells
IF: 0.5-1ug/ml
IHC: 0.5-1ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
IF: 0.5-1ug/ml
IHC: 0.5-1ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
Immunogen
Biotinylated sheep immunoglobulin. Cellular Localization: Depends on the target
Conjugate
FITC
Positive Control
Biotinylated proteins in solution or on tissues
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunohistochemistry (IHC)
(Formalin-paraffin tonsil stained with biotinylated Lambda Light Chain probe followed by Biotin MAb (SPM375). Note cell membrane & cytoplasmic staining.)
Immunohistochemistry (IHC)
(Formalin-paraffin tonsil stained with biotinylated Lambda Light Chain probe followed by Biotin MAb (SPM375). Note cell membrane & cytoplasmic staining.)
Product Categories/Family for anti-Biotin antibody
References
1. Uccellini MB, et al. 2008. J. Immunol. 181:5875. 2. Green NM, et al. 2012. J Biol Chem. 287:39789
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Product Notes
The Biotin (Catalog #AAA6123889) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Biotin can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunofluorescence (IF), Immunohistochemistry (IHC). FC/FACS: 0.5-1ug/million cells IF: 0.5-1ug/ml IHC: 0.5-1ug/ml for 30 minutes at RT Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Biotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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