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Western Blot (WB) (Figure. Western Blot Sample: Recombinant CTNNb1, Human.)

Mouse anti-Human, Rat Beta Catenin (beta-catenin) Monoclonal Antibody | anti-bCat antibody

Anti-Beta Catenin (beta-catenin) Monoclonal Antibody

Gene Names
CTNNB1; EVR7; CTNNB; MRD19; NEDSDV; armadillo
Reactivity
Human, Rat
Applications
Western Blot
Purity
Protein A + Protein G Affinity Chromatography
Synonyms
Beta Catenin (beta-catenin); Monoclonal Antibody; Anti-Beta Catenin (beta-catenin) Monoclonal Antibody; CTNNb1; bCat; b-Cat; CTNN-B1; Catenin Beta 1; Cadherin-Associated Protein; anti-bCat antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Specificity
The antibody is a mouse monoclonal antibody raised against beta-catenin. It has been selected for its ability to recognize beta-catenin in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G Affinity Chromatography
Form/Format
Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
781
Applicable Applications for anti-bCat antibody
Western Blot (WB)
Application Notes
WB: 1:500-2000
Loading Control of Western Blot (WB)
Conjugation
None
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Figure. Western Blot Sample: Recombinant CTNNb1, Human.)

Western Blot (WB) (Figure. Western Blot Sample: Recombinant CTNNb1, Human.)

Western Blot (WB)

(Western Blot Lane1: Mouse Liver TissueLane2: Mouse Lung TissuePrimary Ab: 1:800 Dilution of Mouse Anti-Human CTNNb1 AbSecond Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab)

Western Blot (WB) (Western Blot Lane1: Mouse Liver TissueLane2: Mouse Lung TissuePrimary Ab: 1:800 Dilution of Mouse Anti-Human CTNNb1 AbSecond Ab: 1:5000 Dilution of HRP-Linked Rabbit Anti-Mouse IgG Ab)
Product Categories/Family for anti-bCat antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
beta catenin
NCBI Official Synonym Full Names
catenin beta 1
NCBI Official Symbol
CTNNB1
NCBI Official Synonym Symbols
EVR7; CTNNB; MRD19; NEDSDV; armadillo
NCBI Protein Information
catenin beta-1

NCBI Description

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Research Articles on bCat

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Product Notes

The bCat (Catalog #AAA2111495) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Beta Catenin (beta-catenin) Monoclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Beta Catenin (beta-catenin) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-2000 Loading Control of Western Blot (WB). Researchers should empirically determine the suitability of the bCat for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Beta Catenin (beta-catenin), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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