Human Integrin alpha-V ELISA Kit | ITGAV elisa kit

Human Integrin alpha-V ELISA Kit

Cat. Num. AAA763424

• Gene Names: ITGAV; CD51; MSK8; VNRA; VTNR
• Reactivity: Human
• Synonyms: Integrin alpha-V; Human Integrin alpha-V ELISA Kit; ITGAV (Integrin alpha-V)/CD51/MSK8/Vitronectin Receptor alpha/VNRA/antigen identified by monoclonal L230/CD51 antigen//integrin; alpha V (vitronectin receptor; alpha polypeptide; antigen CD51)/Vitronectin receptor subunit alpha/; ITGAV elisa kit

• Reactivity: Human
• Sequence Length: 1002

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ITGAV elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human ITGAV antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human ITGAV antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ITGAV amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of human ITGAV can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 223468595
• NCBI GeneID: 3685
• NCBI Accession #: NP_001138471.1
• NCBI GenBank Nucleotide #: NM_001144999.2
• Molecular Weight: 111,132 Da
• NCBI Official Full Name: integrin alpha-V isoform 2
• NCBI Official Synonym Full Names: integrin subunit alpha V
• NCBI Official Symbol: ITGAV
• NCBI Official Synonym Symbols: CD51; MSK8; VNRA; VTNR
• NCBI Protein Information: integrin alpha-V
• UniProt Protein Name: Integrin alpha-V
• Protein Family: Integrin
• UniProt Gene Name: ITGAV
• UniProt Synonym Gene Names: MSK8; VNRA
• UniProt Entry Name: ITAV_HUMAN

NCBI Description

The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes. [provided by RefSeq, Oct 2015]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 2q31-q32

Cellular Component: cell surface; external side of plasma membrane; filopodium membrane; focal adhesion; integral to plasma membrane; integrin complex; membrane; microvillus membrane; phagocytic vesicle; plasma membrane

Molecular Function: extracellular matrix binding; fibronectin binding; insulin-like growth factor I binding; metal ion binding; opsonin binding; protease binding; protein binding; protein kinase C binding; transforming growth factor beta binding; viral receptor activity; voltage-gated calcium channel activity

Biological Process: angiogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of peptide antigen via MHC class I; apoptotic cell clearance; axon guidance; blood coagulation; cell adhesion; cell growth; cell migration; cell-matrix adhesion; cell-substrate adhesion; entry of symbiont into host cell by promotion of host phagocytosis; entry of virus into host cell; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte migration; negative chemotaxis; negative regulation of lipid transport; negative regulation of lipoprotein metabolic process; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of osteoblast proliferation; regulation of phagocytosis; vascular endothelial growth factor receptor signaling pathway