Immunfluorescence (IF)/Immunocytochemistry (ICC)
(ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Mouse anti-Human beta-Actin Monoclonal Antibody | anti-ACTB antibody
Human beta-Actin antibody
Immunfluorescence (IF)/Immunocytochemistry (ICC)
(ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Immunfluorescence (IF)/Immunocytochemistry (ICC)
(ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysateLane 3.: PC3 cell lysateLane 4.: HepG2 cell lysateLane 5.: MCF7 cell lysateLane 6.: U87-MG cell lysateLane 7.: NIH-3T3 cell lysate)
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: Jurkat cell lysateLane 3.: PC3 cell lysateLane 4.: HepG2 cell lysateLane 5.: MCF7 cell lysateLane 6.: U87-MG cell lysateLane 7.: NIH-3T3 cell lysate)
Western Blot (WB)
(The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Kidney tissue lysateLane 2.: Liver tissue lysateLane 3.: Spleen tissue lysateLane 4.: Bone marrow tissue lysateLane 5.: Stomach tissue lysate )
Western Blot (WB)
(The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Kidney tissue lysateLane 2.: Liver tissue lysateLane 3.: Spleen tissue lysateLane 4.: Bone marrow tissue lysateLane 5.: Stomach tissue lysate )
Western Blot (WB)
(The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ACTB antibody 1:1000Lane 2.: ACTB antibody 1:3000Lane 3.: ACTB antibody 1:5000Lane 4.: ACTB antibody 1:10000)
Western Blot (WB)
(The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: ACTB antibody 1:1000Lane 2.: ACTB antibody 1:3000Lane 3.: ACTB antibody 1:5000Lane 4.: ACTB antibody 1:10000)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]
Research Articles on ACTB
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Product Notes
The ACTB actb (Catalog #AAA201245) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Human beta-Actin antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's beta-Actin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the ACTB actb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-Actin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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