Mouse beta-Actin Monoclonal Antibody | anti-ACTB antibody

Anti-beta-Actin antibody (monoclonal)

Cat. Num. AAA175084

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken
• Applications: Western Blot, Immunohistochemistry
• Synonyms: beta-Actin; Monoclonal Antibody; Anti-beta-Actin antibody (monoclonal); Actin; cytoplasmic 1; actin; beta; BRWS1; PS1TP5BP1; BETA-ACTIN; ACTIN; CYTOPLASMIC; 1; anti-ACTB antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Pig, Monkey, Rabbit, Chicken
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: AC-15
• Form/Format: Lyophilized; Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
• Sequence Length: 375

• Applicable Applications for anti-ACTB antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin
• Application Notes: Western Blot:; Concentration:0.05-0.01ug/mL; Tested Species: Human, Mouse, Rat, Pig, Monkey, Chicken; ; Immunohistochemistry:; Concentration: 0.5-1ug/mL; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat; ; ; Other applications have not been tested.; ; Optimal dilutions should be determined by end users
• Immunogen: Slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.
• Reconstitution: Add 1 mL of PBS buffer will yield a concentration of 100ug/mL
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Anti-beta-Actin antibody (monoclonal), MBS175084, Western blottingLane 1: Rat Ovary Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: Rat Cardiac Muscle Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: A453 Cell LysateLane 6: HE )

Western Blot (WB)

(Anti-beta-Actin antibody (monoclonal), MBS175084, Western blottingLane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: Rat Kidney Tissue LysateLane 5: HELA Cell LysateLane 6: SMMC Cell )

Immunohistochemistry (IHC)

(Anti-beta-Actin antibody (monoclonal), MBS175084, IHC(P)IHC(P): Mouse Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-beta-Actin antibody (monoclonal), MBS175084, IHC(P)IHC(P): Rat Intestine Tissue )

Immunohistochemistry (IHC)

(Anti-beta-Actin antibody (monoclonal), MBS175084, IHC(P)IHC(P): Human Intestinal Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-beta-Actin antibody (monoclonal), MBS175084, IHC(P)IHC(P): Human Lung Cancer Tissue )

Immunohistochemistry (IHC)

(Anti-beta-Actin antibody (monoclonal), MBS175084, IHC(P)IHC(P): Human Mammary Cancer Tissue )

Related Product Information for anti-ACTB antibody

Description: Mouse IgG monoclonal antibody for beta-Actin, actin, beta (ACTB) detection. Tested with WB, IHC-P in Human, mouse, rat. No cross reactivity with other proteins. Uniprot ID: P60709
Background: The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

NCBI and Uniprot Product Information

• NCBI GI #: 46397333
• NCBI GeneID: 60
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• NCBI Official Full Name: Actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin, beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Beta-actin-like protein
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Uniprot Description

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; postsynaptic density; cytosol; ribonucleoprotein complex; nucleoplasm; cytoskeleton; membrane; axon; nuclear chromatin; cytoplasm; plasma membrane

Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; kinesin binding; Tat protein binding; nitric-oxide synthase binding; protein kinase binding; ATP binding

Biological Process: intercellular junction assembly and maintenance; axon guidance; establishment and/or maintenance of chromatin architecture; protein folding; 'de novo' posttranslational protein folding; retinal homeostasis; cellular protein metabolic process; substantia nigra development; ephrin receptor signaling pathway; ATP-dependent chromatin remodeling; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; cell motility

Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1