Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human non-Hodgkin's Lymphoma stained with Bcl-2 Monoclonal Antibody (SPM530).)
Mouse Bcl-2 Monoclonal Antibody | anti-BCL2 antibody
Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Mouse Monoclonal Antibody
Does not react with Mouse and Rat
Does not react with Mouse and Rat
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human non-Hodgkin's Lymphoma stained with Bcl-2 Monoclonal Antibody (SPM530).)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human non-Hodgkin's Lymphoma stained with Bcl-2 Monoclonal Antibody (SPM530).)
Flow Cytometry (FC/FACS
(Flow Cytometry of Jurkat cells using Bcl-2 Monoclonal Antibody (SPM530) (red) and isotype control (green).)
Flow Cytometry (FC/FACS
(Flow Cytometry of Jurkat cells using Bcl-2 Monoclonal Antibody (SPM530) (red) and isotype control (green).)
Western Blot (WB)
(Western Blot of Bcl-2 in human Skin using Bcl-2 Monoclonal Antibody (SPM530).)
Western Blot (WB)
(Western Blot of Bcl-2 in human Skin using Bcl-2 Monoclonal Antibody (SPM530).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]
Uniprot Description
Bcl-2: a antiapoptotic member of the Bcl-2 family. Regulates cell death by controlling the mitochondrial membrane permeability. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Phosphorylation by JNKs may increase its antiapoptotic functions.
Protein type: Oncoprotein; Apoptosis; Membrane protein, integral; Autophagy
Chromosomal Location of Human Ortholog: 18q21.3
Cellular Component: cytoplasm; endoplasmic reticulum; membrane; mitochondrial outer membrane; mitochondrion; nuclear membrane; nucleus; pore complex
Molecular Function: BH3 domain binding; channel activity; channel inhibitor activity; identical protein binding; protease binding; protein binding; protein heterodimerization activity; protein homodimerization activity; sequence-specific DNA binding; ubiquitin protein ligase binding
Biological Process: apoptosis; B cell proliferation; B cell receptor signaling pathway; defense response to virus; DNA damage response, signal transduction resulting in induction of apoptosis; endoplasmic reticulum calcium ion homeostasis; humoral immune response; induction of apoptosis via death domain receptors; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of cellular pH reduction; negative regulation of mitochondrial depolarization; negative regulation of neuron apoptosis; neuron apoptosis; positive regulation of B cell proliferation; positive regulation of cell growth; protein polyubiquitination; regulation of calcium ion transport; regulation of mitochondrial membrane permeability; regulation of mitochondrial membrane potential; regulation of protein heterodimerization activity; regulation of protein homodimerization activity; regulation of transmembrane transporter activity; release of cytochrome c from mitochondria; response to cytokine stimulus; response to DNA damage stimulus; response to drug; response to iron ion; response to nicotine; response to toxin
Research Articles on BCL2
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Product Notes
The BCL2 bcl2 (Catalog #AAA438104) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Bcl-2 (Apoptosis & Follicular Lymphoma Marker) Mouse Monoclonal Antibody reacts with Human. Does not react with Mouse and Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bcl-2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1ug/ml) Western Blot (0.5-1ug/ml) Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the BCL2 bcl2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bcl-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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