Rabbit Bax Monoclonal Antibody | anti-Bax antibody

Bax mAb

Cat. Num. AAA435296

• Gene Names: BAX; BCL2L4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Synonyms: Bax; Monoclonal Antibody; Bax mAb; Apoptosis regulator BAX; BAX; Bcl-2-like protein 4; BCL2-associated X protein; Bcl2-L-4; BCL2L4; anti-Bax antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 221

• Applicable Applications for anti-Bax antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:1000-1:2000; IHC: 1:50-1:200; IP: 1:50; FC/FACS: 1:50
• Source Note: This monoclonal antibody is produced by immunizing animals with a polypeptide corresponding to Bax protein.
• Preparation and Storage: Store at +4 degree C short term. Store at -20 degree C long term. Avoid freeze / thaw cycle.

Western Blot (WB)

Immunohistochemistry (IHC)

Related Product Information for anti-Bax antibody

Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.

Product Categories/Family for anti-Bax antibody

Apoptosis; BCL2 family Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 612149787
• NCBI GeneID: 581
• NCBI Accession #: NP_001278357.1
• NCBI GenBank Nucleotide #: NM_001291428.1
• UniProt Accession #: Q07812; P55269; Q07814; Q07815; Q8WZ49; Q9NR76; Q9NYG7; Q9UCZ6; Q9UCZ7; Q9UQD6; A8K4W1
• Molecular Weight: 21kDa
• NCBI Official Full Name: apoptosis regulator BAX isoform 1
• NCBI Official Synonym Full Names: BCL2 associated X, apoptosis regulator
• NCBI Official Symbol: BAX
• NCBI Official Synonym Symbols: BCL2L4
• NCBI Protein Information: apoptosis regulator BAX
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Bax inhibitor
• UniProt Gene Name: BAX
• UniProt Synonym Gene Names: BCL2L4; Bcl2-L-4

NCBI Description

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed:21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.