Mouse Costimulatory molecules B7H4  ELISA Kit | B7-H4 elisa kit

Mouse Costimulatory molecules B7H4  ELISA Kit

Cat. Num. AAA733895

• Gene Names: VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• Reactivity: Mouse
• Synonyms: Costimulatory molecules B7H4 ; Mouse Costimulatory molecules B7H4 ELISA Kit; B7-H4 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This B7-H4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse B7-H4. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for B7-H4 elisa kit

Principle of the assay: B7-H4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-B7-H4 antibody and an B7-H4-HRP conjugate. The assay sample and buffer are incubated together with B7-H4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B7-H4 concentration since B7-H4 from samples and B7-H4-HRP conjugate compete for the anti-B7-H4 antibody binding site. Since the number of sites is limited, as more sites are occupied by B7-H4 from the sample, fewer sites are left to bind B7-H4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B7-H4 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 70997756
• NCBI GeneID: 79679
• Molecular Weight: 20,743 Da
• NCBI Official Full Name: B7-H4
• NCBI Official Synonym Full Names: V-set domain containing T cell activation inhibitor 1
• NCBI Official Symbol: VTCN1
• NCBI Official Synonym Symbols: B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291
• NCBI Protein Information: V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4
• UniProt Protein Name: V-set domain-containing T-cell activation inhibitor 1
• UniProt Gene Name: VTCN1
• UniProt Synonym Gene Names: B7H4; UNQ659/PRO1291
• UniProt Entry Name: VTCN1_HUMAN

NCBI Description

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: integral to membrane; external side of plasma membrane

Molecular Function: receptor binding

Biological Process: response to protozoan; immune system process; positive regulation of T cell proliferation; negative regulation of T cell activation