Mouse anti-Rat b2-Microglobulin Monoclonal Antibody | anti-HLA-G antibody

b2-Microglobulin (B2M)

Cat. Num. AAA6000293

• Gene Names: HLA-G; MHC-G
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: b2-Microglobulin; Monoclonal Antibody; b2-Microglobulin (B2M); Anti -b2-Microglobulin (B2M); anti-HLA-G antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 12K137
• Specificity: Recognizes rat B2-Microglobulin. No crossreactivity with recombinant rat alpha-2Macroglobulin.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with PBS to 0.5mg/ml.

• Applicable Applications for anti-HLA-G antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 2.5ug labels 10e6 cells
• Immunogen: Recombinant corresponding to aa21-119 from rat B2-Microglobulin expressed in NSO cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Detection of Rat b2 Microglobulin in Rat Splenocytes. Rat splenocytes were stained with Rat CD3 FITC Cconjugated Monoclonal and either (A) Mouse IgM Isotype control or (B) MBS6000293 followed by Allophycocyanin conjugated Mouse IgM Secondary.)

Related Product Information for anti-HLA-G antibody

B2 Microglobulin (B2M) is a ubiquitous, 12kD, secreted, non-glycosylated protein required for cell surface expression and noncovalent assembly of MHC Class I molecules and CD1 cell surface glycoproteins. Mature rat B2M is a 99aa peptide containing one C1-type lg-like domain (aa22-116). In humans, B2M is known to dissociate from the MHC complex and circulate as full-length and N-terminal truncated peptides of 93, 91, and 90aa. Mature rat B2M is 86% and 75% identical to the corresponding mouse and human protein sequences, respectively.

Product Categories/Family for anti-HLA-G antibody

Antibodies; Abs to Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 188468
• NCBI GeneID: 3135
• UniProt Accession #: P17693
• Molecular Weight: 38,224 Da
• NCBI Official Full Name: b2 microglobulin
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
• UniProt Protein Name: HLA class I histocompatibility antigen, alpha chain G
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Synonym Gene Names: HLA-6.0; HLAG
• UniProt Entry Name: HLAG_HUMAN

NCBI Description

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008]

Uniprot Description

HLA-G: Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T-lymphocytes, macrophages and mononuclear cells. Belongs to the MHC class I family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: Golgi membrane; phagocytic vesicle membrane; membrane; early endosome membrane; plasma membrane; MHC class I protein complex

Molecular Function: protein homodimerization activity; peptide antigen binding; receptor binding

Biological Process: immune response-inhibiting cell surface receptor signaling pathway; regulation of immune response; positive regulation of regulatory T cell differentiation; positive regulation of interleukin-12 production; positive regulation of T cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; negative regulation of immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of tolerance induction; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of T cell proliferation; antigen processing and presentation of exogenous peptide antigen via MHC class I; cellular defense response; positive regulation of T cell tolerance induction

Disease: Asthma, Susceptibility To