Mouse anti-Human ATP6V1B2 Monoclonal Antibody | anti-ATP6V1B2 antibody

Anti-ATP6V1B2 Antibody (clone 1E11)

Cat. Num. AAA2401545

• Gene Names: ATP6V1B2; DOOD; HO57; VATB; VPP3; Vma2; ZLS2; ATP6B2; ATP6B1B2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein A/G purified
• Synonyms: ATP6V1B2; Monoclonal Antibody; Anti-ATP6V1B2 Antibody (clone 1E11); Mouse Monoclonal [clone 2A5] (IgG1; k) to Human ATP6V1B2; ATP6B1B2; ATP6B2; V-ATPase subunit B 2; Vma2; VATB; VPP3; HO57; V-ATPase B2 subunit; anti-ATP6V1B2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1E11
• Specificity: Human ATP6V1B2
• Purity/Purification: Protein A/G purified
• Form/Format: PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
• Sequence Length: 511

• Applicable Applications for anti-ATP6V1B2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 1:100-1:150; WB: 1:2000
• Immunogen: Full length human Recombinant protein of human ATP6V1B2 (NP_001684) produced in HEK293T cell.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ATP6V1B2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ATP6V1B2.)

Related Product Information for anti-ATP6V1B2 antibody

ATP6V1B2 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain.

Product Categories/Family for anti-ATP6V1B2 antibody

Transporter; ATPase - V type

NCBI and Uniprot Product Information

• NCBI GI #: 19913428
• NCBI GeneID: 526
• NCBI Accession #: NP_001684.2
• NCBI GenBank Nucleotide #: NM_001693.3
• UniProt Accession #: P21281; Q14544; Q15859; Q96IR0; B2R5Z3; D3DSQ5
• Molecular Weight: 56,501 Da
• NCBI Official Full Name: V-type proton ATPase subunit B, brain isoform
• NCBI Official Synonym Full Names: ATPase H+ transporting V1 subunit B2
• NCBI Official Symbol: ATP6V1B2
• NCBI Official Synonym Symbols: DOOD; HO57; VATB; VPP3; Vma2; ZLS2; ATP6B2; ATP6B1B2
• NCBI Protein Information: V-type proton ATPase subunit B, brain isoform
• UniProt Protein Name: V-type proton ATPase subunit B, brain isoform
• Protein Family: V-type proton ATPase
• UniProt Gene Name: ATP6V1B2
• UniProt Synonym Gene Names: ATP6B2; VPP3; V-ATPase subunit B 2

NCBI Description

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]

Uniprot Description

Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.