Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ATP6V1B2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Rabbit anti-Mouse, Rat ATP6V1B2 Polyclonal Antibody | anti-ATP6V1B2 antibody
ATP6V1B2 Rabbit pAb
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ATP6V1B2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ATP6V1B2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts. [provided by RefSeq, Jul 2008]
Uniprot Description
ATP6V1B2: Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the ATPase alpha/beta chains family.
Protein type: EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase
Chromosomal Location of Human Ortholog: 8p21.3
Cellular Component: ruffle; microvillus; intracellular membrane-bound organelle; lysosomal membrane; integral to membrane; melanosome; plasma membrane; endomembrane system; cytosol
Molecular Function: hydrogen ion transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding
Biological Process: ATP metabolic process; interaction with host; proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; insulin receptor signaling pathway; transferrin transport; transmembrane transport
Disease: Zimmermann-laband Syndrome 2
Research Articles on ATP6V1B2
Similar Products
Product Notes
The ATP6V1B2 atp6v1b2 (Catalog #AAA9142451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATP6V1B2 Rabbit pAb reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATP6V1B2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the ATP6V1B2 atp6v1b2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: NLANDPTIER IITPRLALTT AEFLAYQCEK HVLVILTDMS SYAEALREVS AAREEVPGRR GFPGYMYTDL ATIYERAGRV EGRNGSITQI PILTMPNDDI THPIPDLTGY ITEGQIYVDR QLHNRQIYPP INVLPSLSRL MKSAIGEGMT RKDHADVSNQ LYACYAIGKD VQAMKAVVGE EALTSDDLLY LEFLQKFERN FIAQGPYENR TVFETLDIGW QLLRIFPKEM LKRIPQSTLS EFYPRDSAKH. It is sometimes possible for the material contained within the vial of "ATP6V1B2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
