Mouse anti-Human AT Rich Interactive Domain 3A Monoclonal Antibody | anti-ARID3A antibody

AT Rich Interactive Domain 3A (BRIGHT-Like, ARID Domain-Containing Protein 3A, Dead Ringer-like Protein 1)

Cat. Num. AAA601972

• Gene Names: ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: AT Rich Interactive Domain 3A; Monoclonal Antibody; AT Rich Interactive Domain 3A (BRIGHT-Like; ARID Domain-Containing Protein 3A; Dead Ringer-like Protein 1); Anti -AT Rich Interactive Domain 3A (BRIGHT-Like; anti-ARID3A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C24
• Specificity: Recognizes human AT Rich Interactive Domain 3A.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-ARID3A antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human AT Rich Interactive Domain 3A
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Western Blot (WB)

(Detection of human ARID3A by Western blot. Samples: Whole cell lysate (25 μg) from HEK293 cells. Predicted molecular weight: 62 kD)

Product Categories/Family for anti-ARID3A antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 119589986
• NCBI GeneID: 1820
• UniProt Accession #: Q99856; Q5I858; Q6P9C6; Q8IZA7; Q8N4Z3
• Molecular Weight: 62,889 Da
• NCBI Official Full Name: AT rich interactive domain 3A (BRIGHT- like)
• NCBI Official Synonym Full Names: AT rich interactive domain 3A (BRIGHT-like)
• NCBI Official Symbol: ARID3A
• NCBI Official Synonym Symbols: DRIL1; DRIL3; BRIGHT; E2FBP1
• NCBI Protein Information: AT-rich interactive domain-containing protein 3A; dead ringer-like 1; E2F-binding protein 1; ARID domain-containing 3A; dead ringer-like protein 1; ARID domain-containing protein 3A; B-cell regulator of IgH transcription; AT rich interactive domain 3A (BRIGHT- like) protein
• UniProt Protein Name: AT-rich interactive domain-containing protein 3A
• Protein Family: AT-rich interactive domain-containing protein
• UniProt Gene Name: ARID3A
• UniProt Synonym Gene Names: DRIL1; DRIL3; DRX; E2FBP1
• UniProt Entry Name: ARI3A_HUMAN

NCBI Description

This gene encodes a member of the ARID (AT-rich interaction domain) family of DNA binding proteins. It was found by homology to the Drosophila dead ringer gene, which is important for normal embryogenesis. Other ARID family members have roles in embryonic patterning, cell lineage gene regulation, cell cycle control, transcriptional regulation, and possibly in chromatin structure modification. [provided by RefSeq]

Uniprot Description

Bright: a member of the ARID (AT-rich interaction domain) family of transcription factors. It was found by its homology to the Drosophila dead ringer protein, which is important for normal embryogenesis. Binds a VH promoter proximal site necessary for induced mu-heavy-chain transcription. Binds the minor groove of a restricted ATC sequence that is sufficient for nuclear matrix association. This sequence motif is present in matrix-associating regions (MARS) proximal to the promoter and flanking E mu. Activates E mu-driven transcription by binding these sites. Contributes to localized control of accessibility and therefore nonrandom gene use during V(D)J recombination.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; nucleolus; lipid raft

Molecular Function: protein binding; protein homodimerization activity; chromatin binding

Biological Process: transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter