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Western Blot (WB)
(Western blot analysis of DSTN Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DSTN (arrow) was detected using the purified Pab.)
Rabbit anti-Human DSTN Polyclonal Antibody | anti-DSTN antibody
DSTN, ID (DSTN, ACTDP, DSN, Destrin, Actin-depolymerizing factor) (AP)
Gene Names
DSTN; ADF; ACTDP; bA462D18.2
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DSTN; Polyclonal Antibody; ID (DSTN; ACTDP; DSN; Destrin; Actin-depolymerizing factor) (AP); Actin-depolymerizing factor; anti-DSTN antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-DSTN antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-100
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DSTN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77~106 amino acids from the Center region of human DSTN.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of DSTN Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DSTN (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of DSTN Antibody (Center) in MCF-7 cell line lysates (35ug/lane). DSTN (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with DSTN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with DSTN Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(DSTN Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DSTN Antibody (Center) flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB)
(Western blot analysis of DSTN (arrow) using rabbit polyclonal DSTN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DSTN gene.)
Western Blot (WB)
(Western blot analysis of DSTN (arrow) using rabbit polyclonal DSTN Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DSTN gene.)
Related Product Information for anti-DSTN antibody
DSTN belongs to the actin-binding proteins ADF family. This family of proteins is responsible for enhancing the turnover rate of actin in vivo. This protein encodes the actin depolymerizing protein that severs actin filaments (F-actin) and binds to actin monomers (G-actin).
Product Categories/Family for anti-DSTN antibody
References
Estornes, Y., et.al., Int. J. Cancer 121 (10), 2162-2171 (2007). Mannherz, H.G., et.al., J. Mol. Biol. 366 (3), 745-755 (2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
165
NCBI Official Full Name
destrin isoform b
NCBI Official Synonym Full Names
destrin (actin depolymerizing factor)
NCBI Official Symbol
DSTN
NCBI Official Synonym Symbols
ADF; ACTDP; bA462D18.2
NCBI Protein Information
destrin; actin-depolymerizing factor; bA462D18.2 (destrin (actin depolymerizing factor ADF) (ACTDP))
UniProt Protein Name
Destrin
Protein Family
UniProt Gene Name
DSTN
UniProt Synonym Gene Names
ACTDP; DSN; ADF
UniProt Entry Name
DEST_HUMAN
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Product Notes
The DSTN dstn (Catalog #AAA6293609) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DSTN, ID (DSTN, ACTDP, DSN, Destrin, Actin-depolymerizing factor) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DSTN can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-100 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DSTN dstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DSTN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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