Western Blot (WB)
(Western Blot:Sample: Recombinant ALOX5, Human.)
Mouse Arachidonate-5-Lipoxygenase (ALOX5) Monoclonal Antibody | anti-ALOX5 antibody
Monoclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot:Sample: Recombinant ALOX5, Human.)
Western Blot (WB)
(Western Blot:Sample: Recombinant ALOX5, Human.)
Western Blot (WB)
(Western Blot;Sample: Human Placenta lysate;Primary Ab: 29g/ml Mouse Anti-Human ALOX5 AntibodySecond Ab: 0.29g/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
Western Blot (WB)
(Western Blot;Sample: Human Placenta lysate;Primary Ab: 29g/ml Mouse Anti-Human ALOX5 AntibodySecond Ab: 0.29g/mL HRP-Linked Rabbit Anti-Mouse IgG Polyclonal Antibody (Catalog: MBS2086035))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]
Uniprot Description
ALOX15: Converts arachidonic acid to 15S- hydroperoxyeicosatetraenoic acid. Also acts on C-12 of arachidonate as well as on linoleic acid. Belongs to the lipoxygenase family.
Protein type: EC 1.13.11.31; EC 1.13.11.33; Lipid Metabolism - arachidonic acid; Lipid Metabolism - linoleic acid; Motility/polarity/chemotaxis; Oxidoreductase
Chromosomal Location of Human Ortholog: 17p13.2
Cellular Component: cytosol; extrinsic component of cytoplasmic side of plasma membrane; lipid droplet; membrane; plasma membrane
Molecular Function: arachidonate 12-lipoxygenase activity; arachidonate 15-lipoxygenase activity; hepoxilin A3 synthase activity; hepoxilin-epoxide hydrolase activity; iron ion binding; phosphatidylinositol-4,5-bisphosphate binding; protein binding
Biological Process: apoptotic cell clearance; arachidonic acid metabolic process; bone mineralization; cellular response to calcium ion; cellular response to interleukin-13; hepoxilin biosynthetic process; inflammatory response; leukotriene metabolic process; lipoxin A4 biosynthetic process; lipoxygenase pathway; negative regulation of adaptive immune response; ossification; phosphatidylethanolamine biosynthetic process; positive regulation of actin filament polymerization; positive regulation of cell-substrate adhesion; positive regulation of ERK1 and ERK2 cascade; positive regulation of heterotypic cell-cell adhesion; regulation of engulfment of apoptotic cell; wound healing
Research Articles on ALOX5
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Product Notes
The ALOX5 alox15 (Catalog #AAA2001302) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Arachidonate-5-Lipoxygenase (ALOX5) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Arachidonate-5-Lipoxygenase (ALOX5) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the ALOX5 alox15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arachidonate-5-Lipoxygenase (ALOX5), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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