Apolipoprotein AI (Apo AI) Monoclonal Antibody | anti-APOA1 antibody

MAb to Apolipoprotein AI

Cat. Num. AAA310967

• Gene Names: APOA1; MGC117399
• Purity: Protein A chromatography
• Synonyms: Apolipoprotein AI (Apo AI); Monoclonal Antibody; MAb to Apolipoprotein AI; Monoclonal Antibody to Human Apolipoprotein A1 (Apo AI); anti-APOA1 antibody

• Host: Host: Mouse. Hybridization of P3X63-Ag 8.653 myeloma cells with spleen cells from BALB/c mice.; Source: Ascites
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1405
• Specificity: Apolipoprotein AI (Apo AI); Recognizes human apolipoprotein AI. No cross reactivity with Apo A-II or Apo B
• Purity/Purification: Protein A chromatography
• Form/Format: Purified, Liquid
• Concentration: 3.47mg/ml (OD280nm, E^1% = 14) (varies by lot)
• Sequence Length: 267

• Immunogen: Purified human plasma Apo A-I
• Affinity Constant: 8 x 10^9
• Buffer: PBS, pH 7.0
• Preservative: 0.02% Sodium azide
• Important Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Warnings: This product contains sodium azide, which has been classified as Xn (Harmful), in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.
• Preparation and Storage: Short-term (up to 6 months) store at 2 to 8 degree C. Long term (up to 1 year), aliquot and store at -20 degree C. Avoid multiple freeze/thaw cycles.

Product Categories/Family for anti-APOA1 antibody

Monoclonal Antibodies to Lipoproteins

References

• Jamil, H., (1996), "An inhibitor of the microsomal triglyceride transfer protein inhibits ApoB secretion from HepG2 cells", Proc. Natl. Acad. Sci. USA, 93, 11991-11995
• Ishiguro, H., et al., (2001), "Retrovirus-mediated Expressio

NCBI and Uniprot Product Information

• NCBI GI #: 296635
• NCBI GeneID: 335
• UniProt Accession #: P02647; Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866; Q6LEJ8; Q8TDB0; Q9Y355
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein AI
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: MGC117399
• NCBI Protein Information: apolipoprotein A-I; apo-AI; apoA-I; OTTHUMP00000043268; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Lipid-binding; Cell development/differentiation; Endoplasmic reticulum; Vesicle

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: extracellular space; chylomicron; cell surface; endocytic vesicle; endoplasmic reticulum lumen; early endosome; plasma membrane; extracellular region; cytoplasmic vesicle; nucleus; cytosol; vesicle

Molecular Function: identical protein binding; protein binding; enzyme binding; phospholipid transporter activity; lipase inhibitor activity; beta-amyloid binding; chemorepellent activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; high-density lipoprotein binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding

Biological Process: phototransduction, visible light; negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; negative regulation of interleukin-1 beta secretion; sequestering of lipid; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; response to drug; cholesterol metabolic process; platelet activation; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; lipoprotein biosynthetic process; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood vessel endothelial cell migration; cellular lipid metabolic process; platelet degranulation; phospholipid efflux; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; positive regulation of fatty acid biosynthetic process; receptor-mediated endocytosis; regulation of protein amino acid phosphorylation; cholesterol transport; protein stabilization; protein amino acid oxidation; negative regulation of heterotypic cell-cell adhesion; neurite regeneration; cholesterol efflux; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; reverse cholesterol transport; endothelial cell proliferation; negative regulation of inflammatory response; blood coagulation

Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral