Human apoprotein A1 ELISA Kit | Apo-A1 elisa kit

Human apoprotein A1 ELISA Kit

Cat. Num. AAA726523

• Gene Names: APOA1; MGC117399
• Reactivity: Human
• Synonyms: apoprotein A1; Human apoprotein A1 ELISA Kit; apoprotein A1 (Human); Apo-A1 elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Apo-A1 elisa kit

Intended Uses: This APLP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human APLP1. This ELISA kit for research use only!

Principle of the Assay: APLP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-APLP1 antibody and an APLP1-HRP conjugate. The assay sample and buffer are incubated together with APLP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APLP1 concentration since APLP1 from samples and APLP1-HRP conjugate compete for the anti-APLP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by APLP1 from the sample, fewer sites are left to bind APLP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APLP1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for Apo-A1 elisa kit

Human ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 4557321
• NCBI GeneID: 335
• NCBI Accession #: NP_000030.1
• NCBI GenBank Nucleotide #: NM_000039.1
• UniProt Accession #: P02647; Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866; Q6LEJ8; Q8TDB0; Q9Y355
• Molecular Weight: 30,778 Da
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Official Synonym Symbols: MGC117399
• NCBI Protein Information: apolipoprotein A-I; apo-AI; apoA-I; OTTHUMP00000043268; OTTHUMP00000069346; OTTHUMP00000069347; OTTHUMP00000069348
• UniProt Protein Name: Apolipoprotein A-I
• UniProt Gene Name: APOA1
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

Uniprot Description

Function: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Ref.20

Subunit structure: Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Ref.25 Ref.31

Subcellular location: Secreted.

Tissue specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. Ref.33

Post-translational modification: Palmitoylated. Ref.28Phosphorylation sites are present in the extracelllular medium.

Involvement in disease: Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [

MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant. Ref.37 Ref.38Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [

MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I. Ref.37 Ref.38Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [

MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed. Ref.37 Ref.38 Ref.43 Ref.44Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [

MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Ref.37 Ref.38 Ref.46

Sequence similarities: Belongs to the apolipoprotein A1/A4/E family.