Mouse anti-Human Cyclin H Monoclonal Antibody | anti-CCNH antibody

Cyclin H antibody

Cat. Num. AAA200367

• Gene Names: CCNH; CAK; p34; p37; CycH
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: By protein-A affinity chromatography
• Synonyms: Cyclin H; Monoclonal Antibody; Cyclin H antibody; CCNH; CAK; p34; p37; 6330408H09Rik; AI661354; AV102684; AW538719; CDK activating kinase; Cyclin dependent kinase activating kinase; CyclinH; MO15 associated protein; P36; Cyclin-H; anti-CCNH antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b, k
• Clone Number: AT3G6
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 323

• Applicable Applications for anti-CCNH antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: WB: 1:1000.
• Antigen Species: Human
• Immunogen: Recombinant human Cyclin H (1-323aa) purified from E Coli
• Clone Note: Anti-human Cyclin H mAb, clone AT3G6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Cyclin H protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Recombinant Human Cyclin H (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Cyclin H antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human Cyclin H Lane 2.: HepG2 cell lysate Lane 3.: Jurkat cell lysate Lane 4 : Ramos cell lysate Lane 5 : Balb/3T3 cell lysate Lane 6 : A431 cell lysate)

Related Product Information for anti-CCNH antibody

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery.

Product Categories/Family for anti-CCNH antibody

Cell Cycle

References

Jeang KT., et al. (1998) J Biomed Sci. 5 (1): 24-27.; Yankulov K., et al. (1998) Current Biology. 8 (13): 447-449.

NCBI and Uniprot Product Information

• NCBI GI #: 4502623
• NCBI GeneID: 902
• NCBI Accession #: NP_001230
• NCBI GenBank Nucleotide #: NM_001239.3
• UniProt Accession #: P51946; Q53X72; Q8TBL9
• Molecular Weight: 37,643 Da
• NCBI Official Full Name: cyclin-H isoform 1
• NCBI Official Synonym Full Names: cyclin H
• NCBI Official Symbol: CCNH
• NCBI Official Synonym Symbols: CAK; p34; p37; CycH
• NCBI Protein Information: cyclin-H
• UniProt Protein Name: Cyclin-H
• Protein Family: Cyclin
• UniProt Gene Name: CCNH

NCBI Description

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with CDK7 kinase and ring finger protein MAT1. The kinase complex is able to phosphorylate CDK2 and CDC2 kinases, thus functions as a CDK-activating kinase (CAK). This cyclin and its kinase partner are components of TFIIH, as well as RNA polymerase II protein complexes. They participate in two different transcriptional regulation processes, suggesting an important link between basal transcription control and the cell cycle machinery. A pseudogene of this gene is found on chromosome 4. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Nov 2010]

Uniprot Description

Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.