Mouse anti-Human ANGPTL5 Monoclonal Antibody | anti-ANGPTL5 antibody

ANGPTL5 (Angiopoietin-related Protein 5, Angiopoietin-like Protein 5, UNQ5795/PRO19600)

Cat. Num. AAA6008182

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ANGPTL5; Monoclonal Antibody; ANGPTL5 (Angiopoietin-related Protein 5; Angiopoietin-like Protein 5; UNQ5795/PRO19600); Anti -ANGPTL5 (Angiopoietin-related Protein 5; anti-ANGPTL5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1B2
• Specificity: Recognizes human ANGPTL5.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MMSPSQASLLFLNVCIFICGEAVQGNCVHHSTDSSVVNIVEDGSNAKDESKSNDTVCKEDCEESCDVKTKITREEKHFMCRNLQNSIVSYTRSTKKLLRNMMDEQQASLDYLSNQVNELMNRVLLLTTEVFRKQLDPFPHRPVQSHGLDCTDIKDTIGSVTKTPSGLYIIHPEGSSYPFEVMCDMDYRGGGWTVIQKRIDGIIDFQRLWCDYLDGFGDLLGEFWLGLKKIFYIVNQKNTSFMLYVALESEDDTLAYASYDNFWLEDETRFFKMHLGRYSGNAGDAFRGLKREDNQNAKPFSTSDVDNDGCRPACLVNGQSVKSCSHLHNKTGWWFNECGLANLNGIHHFSGKLLATGIQWGTWTKNNSPVKIKSVSMKIRRMYNPYFK

• Applicable Applications for anti-ANGPTL5 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Sandwich ELISA: The detection limit is ~10ng/ml as a capture antibody.
• Immunogen: Full length recombinant protein corresponding to aa1-389 from human ANGPTL5 (AAH49170) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (68.79kD).)

Western Blot (WB)

(ANGPTL5 monoclonal antibody. Western Blot analysis of ANGPTL5 expression in human kidney.)

Western Blot (WB)

(Western Blot analysis of ANGPTL5 expression in transfected 293T cell line by ANGPTL5 monoclonal antibody.|Lane 1: ANGPTL5 transfected lysate (44.2kD).|Lane 2: Non-transfected lysate.)

Immunoprecipitation (IP)

(Immunoprecipitation of ANGPTL5 transfected lysate using ANGPTL5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ANGPTL5 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged ANGPTL5 is 10ng/ml as a capture antibody.)

Related Product Information for anti-ANGPTL5 antibody

Angiopoietin-like 5 (ANGPTL5) is a secreted glycoprotein that is structurally related to the angiopoietins which contain an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. It shares 88% and 93% aa sequence identity with bovine and porcine ANGPTL5, and greater than 98% identity with chimpanzee, orangutan, and rhesus ANGPTL5. ANGPTL5 is expressed in adipose, bronchial, epididymal, and heart tissue. Rare polymorphisms and loss of function mutations in human ANGPTL5 are associated with low circulating triglyceride levels and alterations in body mass index. ANGPTL5, when used in combination with other growth factors such as SCF, Thrombopoietin, IGF-II, FGF acidic, Flt-3 Ligand, and IGFBP-2, enhances the expansion and engraftment of human and mouse hematopoietic stem cells. The coiled-coil domain of ANGPTL proteins is critical for this effect. However, the molecular mechanism of ANGPTL proteins in stem cell activity remains unclear.

Product Categories/Family for anti-ANGPTL5 antibody

Antibodies; Abs to Angiopoietin

NCBI and Uniprot Product Information

• NCBI GI #: 187960047
• NCBI GeneID: 253935
• NCBI Accession #: NP_835228.2
• NCBI GenBank Nucleotide #: NM_178127.4
• UniProt Accession #: Q86XS5; Q86VR9; A8K658
• Molecular Weight: 44,144 Da
• NCBI Official Full Name: angiopoietin-related protein 5
• NCBI Official Synonym Full Names: angiopoietin-like 5
• NCBI Official Symbol: ANGPTL5
• NCBI Protein Information: angiopoietin-related protein 5; angiopoietin-like protein 5
• UniProt Protein Name: Angiopoietin-related protein 5
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL5
• UniProt Entry Name: ANGL5_HUMAN

Uniprot Description

ANGPTL5:

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q22.1

Cellular Component: extracellular region