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Western Blot (WB) (The anti-MAGEA9 Ctr Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.)

Rabbit anti-Human MAGEA9 Polyclonal Antibody | anti-MAGEA9 antibody

MAGEA9, ID (Melanoma-associated Antigen 9, MAGE-9 Antigen, Cancer/Testis Antigen 1.9, CT1.9, MAGE9, MAGEA9A) (Azide free) (HRP)

Gene Names
MAGEA9; CT1.9; MAGE9
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
MAGEA9; Polyclonal Antibody; ID (Melanoma-associated Antigen 9; MAGE-9 Antigen; Cancer/Testis Antigen 1.9; CT1.9; MAGE9; MAGEA9A) (Azide free) (HRP); anti-MAGEA9 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MAGEA9.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Applicable Applications for anti-MAGEA9 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
ELISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the center region of human MAGEA9 (KLH).
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-MAGEA9 Ctr Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.)

Western Blot (WB) (The anti-MAGEA9 Ctr Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(Western blot analysis of MAGEA9 (arrow) using rabbit polyclonal MAGEA9 Antibody (C185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEA9 gene.)

Western Blot (WB) (Western blot analysis of MAGEA9 (arrow) using rabbit polyclonal MAGEA9 Antibody (C185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEA9 gene.)
Product Categories/Family for anti-MAGEA9 antibody
References
1. Rogner, U.C., et al., Genomics 29(3):725-731 (1995). 2. De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,088 Da
NCBI Official Full Name
melanoma-associated antigen 9
NCBI Official Synonym Full Names
melanoma antigen family A9
NCBI Official Symbol
MAGEA9
NCBI Official Synonym Symbols
CT1.9; MAGE9
NCBI Protein Information
melanoma-associated antigen 9
UniProt Protein Name
Melanoma-associated antigen 9
UniProt Gene Name
MAGEA9
UniProt Synonym Gene Names
MAGE9; MAGEA9A; CT1.9
UniProt Entry Name
MAGA9_HUMAN

NCBI Description

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

Uniprot Description

MAGE-A9: Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28

Research Articles on MAGEA9

Similar Products

Product Notes

The MAGEA9 magea9 (Catalog #AAA6486268) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAGEA9, ID (Melanoma-associated Antigen 9, MAGE-9 Antigen, Cancer/Testis Antigen 1.9, CT1.9, MAGE9, MAGEA9A) (Azide free) (HRP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEA9 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:50-1:100 ELISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAGEA9 magea9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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