Rabbit MAPK10 Polyclonal Antibody | anti-MAPK10 antibody

MAPK10 Antibody

Cat. Num. AAA2521766

• Gene Names: MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Antigen affinity purification
• Synonyms: MAPK10; Polyclonal Antibody; MAPK10 Antibody; FLJ12099; FLJ33785; JNK3; JNK3A; MGC50974; PRKM10; p493F12; p54Bsapk; anti-MAPK10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 464

• Applicable Applications for anti-MAPK10 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Immunogen: Recombinant protein of human MAPK10
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-46014 / sc-46015
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MAPK10 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using MAPK10 antibody at dilution of 1:200 (x400 lens))

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using MAPK10 antibody at dilution of 1:200 (x400 lens))

Related Product Information for anti-MAPK10 antibody

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing, resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3, 5).

NCBI and Uniprot Product Information

• NCBI GI #: 20986510
• NCBI GeneID: 5602
• NCBI Accession #: NP_620448.1
• NCBI GenBank Nucleotide #: NM_138982.2
• Molecular Weight: 31,933 Da
• NCBI Official Full Name: mitogen-activated protein kinase 10 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 10
• NCBI Official Symbol: MAPK10
• NCBI Official Synonym Symbols: JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
• NCBI Protein Information: mitogen-activated protein kinase 10; JNK3 alpha protein kinase; MAP kinase 10; MAP kinase p49 3F12; MAPK 10; c-Jun N-terminal kinase 3; stress activated protein kinase beta; stress-activated protein kinase 1b; stress-activated protein kinase JNK3
• UniProt Protein Name: Mitogen-activated protein kinase 10
• UniProt Gene Name: MAPK10
• UniProt Synonym Gene Names: JNK3; JNK3A; PRKM10; SAPK1B; MAP kinase 10; MAPK 10; SAPK1b
• UniProt Entry Name: MK10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuronal-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways during neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with, and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 can phosphorylate, and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

JNK3: a protein kinase of the MAPK family that is potently activated by a variety of environmental stress and pro-inflammatory cytokines. Brain-selective JNK isoform. A pro-apoptotic protein and potential tumor suppressor. Phosphorylates a number of transcription factors including c-Jun, ATF2 and ELK1. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity. CDK5 can phosphorylate and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Interacts with HDAC9. Expression lost in brain tumors. May function in neuronal cell death from injury and neurodegeneration, for which inhibitors are being developed. Two alternatively spliced transcript variants have been reported.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily

Chromosomal Location of Human Ortholog: 4q22.1-q23

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; JUN kinase activity; ATP binding

Biological Process: response to light stimulus; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; regulation of circadian rhythm; signal transduction; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; JUN phosphorylation; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; toll-like receptor signaling pathway; innate immune response; JNK cascade; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway