Immunofluorescene (IF)
(MBS9604445 staining NCI-H1299 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Rabbit DRP1 Polyclonal Antibody | anti-DRP1 antibody
Phospho-DRP1 (Ser637) Antibody
IHC: 1:50-1:200
IF: 1:100-1:500
ICC: 1:100-1:500
ELISA(peptide): 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
Important: For western blots, incubate membrane with duluted antibody in 5% w/v milk, 1X TBS , 0.1% TweenR20 at 4°C with gentle shaking , overnight.
Immunofluorescene (IF)
(MBS9604445 staining NCI-H1299 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Immunofluorescene (IF)
(MBS9604445 staining NCI-H1299 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
Immunohistochemistry (IHC)
(MBS9604445 at 1/100 staining Human pancreatic cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Rabbitantibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9604445 at 1/100 staining Human pancreatic cancer by IHC-P.The sample was formaldehyde fixed and a heat mediatedantigen retrieval step in citrate buffer was performed. Thesample was then blocked and incubated with the primaryantibody at 4°C overnight. An HRP conjugated anti-Rabbitantibody was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 whole cell lysates ,using Phospho-DRP1 (Ser637) Antibody. Lane1 was treatedwith phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide)
Western Blot (WB)
(Western blot analysis of extracts from 293 whole cell lysates ,using Phospho-DRP1 (Ser637) Antibody. Lane1 was treatedwith phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide)
Immunohistochemistry (IHC)
(MBS9604445 at 1/100 staining human liver carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604445 at 1/100 staining human liver carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of Phospho-DRP1 (Ser637) using 293 whole cell lysates)
Western Blot (WB)
(Western blot analysis of Phospho-DRP1 (Ser637) using 293 whole cell lysates)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the dynamin superfamily of GTPases. The encoded protein mediates mitochondrial and peroxisomal division, and is involved in developmentally regulated apoptosis and programmed necrosis. Dysfunction of this gene is implicated in several neurological disorders, including Alzheimer's disease. Mutations in this gene are associated with the autosomal dominant disorder, encephalopathy, lethal, due to defective mitochondrial and peroxisomal fission (EMPF). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2013]
Uniprot Description
Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis (PubMed:26992161, PubMed:27301544, PubMed:27328748). Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution.
Research Articles on DRP1
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Product Notes
The DRP1 dnm1l (Catalog #AAA9604445) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-DRP1 (Ser637) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DRP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 IF: 1:100-1:500 ICC: 1:100-1:500 ELISA(peptide): 1:20000-1:40000 *The optimal dilutions should be determined by the end user. Important: For western blots, incubate membrane with duluted antibody in 5% w/v milk, 1X TBS, 0.1% TweenR20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the DRP1 dnm1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DRP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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