Typical Testing Data/Standard Curve (for reference only)
Mouse Connexin 43 ELISA Kit | CX43 elisa kit
Mouse Connexin 43 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CX43 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CX43 antibody and an CX43-HRP conjugate. The assay sample and buffer are incubated together with CX43-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX43 concentration since CX43 from samples and CX43-HRP conjugate compete for the anti-CX43 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX43 from the sample, fewer sites are left to bind CX43-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX43 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
Uniprot Description
Function: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Ref.1Plays a role in regulation of fin bone size and growth. Ref.1
Subunit structure: A connexon is composed of a hexamer of connexins.
Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction.
Tissue specificity: Expressed in adult ovary, heart, lens and liver. In growing caudal fins, expressed in cells flanking the germinal region of newly growing segments and in osteoblasts at segment boundaries. Ref.1 Ref.2 Ref.5
Developmental stage: Expressed both maternally and zygotically. Zygotic expression is initiated before gastrulation and persists through to day 5 of development. Levels increase in late somitogenesis, and late embryonic and early larval stages. Embryonic expression is observed in many developing tissues and organs, including the notochord and brain, and heart and vasculature. Detected throughout the lens at 24 hours post-fertilization (hpf), but by 3-4 days development, restricted to the lateral epithelium and differentiating cells in the equatorial region. Ref.2 Ref.5
Miscellaneous: Morpholino knockdown of the protein results in heart folding defects, reduced hematopoiesis and small eyes.
Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.
Research Articles on CX43
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Product Notes
The Mouse CX43 gja1 (Catalog #AAA729401) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA729401 ELISA Kit recognizes Mouse CX43. It is sometimes possible for the material contained within the vial of "Connexin 43, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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