Mouse Alpha A Crystallin Monoclonal Antibody | anti-CRYAA antibody

Alpha A Crystallin Antibody

Cat. Num. AAA8001894

• Gene Names: CRYAA; CRYA1; HSPB4; CTRCT9
• Reactivity: Human, Mouse, Rat, Bovine
• Applications: Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: Alpha A Crystallin; Monoclonal Antibody; Alpha A Crystallin Antibody; Mouse Anti-Human Alpha A Crystallin Monoclonal IgG1; Clone 1H3.B8; Heat shock protein beta4 Antibody; Acry 1 Antibody; CRYA1 Antibody; CRYAA Antibody; HSPB4 Antibody; anti-CRYAA antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Bovine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 1H3.B8
• Specificity: Detects ~20kDa. Does not cross-react with alphaB-crystallin, betaL-crystallin, betaH-crystallin, gamma-crystallin, HSP25, HSP27 or HSP47 proteins.
• Purity/Purification: Protein G Purified
• Form/Format: PBS pH 7.2, 50% glycerol, 0.09% Sodium Azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 173

• Applicable Applications for anti-CRYAA antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:2000; ICC/IF: 1:100
• Immunogen: Native Alpha Crystallin
• Immunogen Species: Human
• Conjugate: Unconjugated
• Cellular Localization: Cytoplasm, Nucleus
• Certificate of Analysis: 0.5 ug/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.
• Preparation and Storage: Store at-20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha A Crystallin Monoclonal Antibody, Clone 1H3.B8 . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha A Crystallin Antibody. (D) Composite.)

Western Blot (WB)

(Western Blot analysis of Bovine tissue lysate showing detection of Alpha A Crystallin protein using Mouse Anti-Alpha A Crystallin Monoclonal Antibody, Clone 1H3.B8 . Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Alpha A Crystallin Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. This blot shows absolute specificity as Left: Alpha A Crystallin, Right: Alpha B Crystallin.)

Related Product Information for anti-CRYAA antibody

The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).

Product Categories/Family for anti-CRYAA antibody

Cancer; Heat Shock; Cell Signaling; Protein Trafficking; Chaperone Proteins; Neuroscience

References

1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052. 2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453. 3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490 4. Horwitz J. (2003) Exp Eye Res. 76: 145-153. 5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924. 6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377. 7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215. 8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.

NCBI and Uniprot Product Information

• NCBI GI #: 4503055
• NCBI GeneID: 1409
• NCBI Accession #: NP_000385.1
• NCBI GenBank Nucleotide #: NM_000394.3
• UniProt Accession #: P02489
• NCBI Official Full Name: alpha-crystallin A chain isoform 1
• NCBI Official Synonym Full Names: crystallin alpha A
• NCBI Official Symbol: CRYAA
• NCBI Official Synonym Symbols: CRYA1; HSPB4; CTRCT9
• NCBI Protein Information: alpha-crystallin A chain
• UniProt Protein Name: Alpha-crystallin A chain
• Protein Family: Alpha-crystallin
• UniProt Gene Name: CRYAA
• UniProt Synonym Gene Names: CRYA1; HSPB4; HspB4
• UniProt Entry Name: CRYAA_HUMAN

NCBI Description

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]

Uniprot Description

CRYA1: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP, also known as the HSP20) family. Alpha-A is preferentially restricted to the lens.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: cytoplasm; nucleus

Molecular Function: identical protein binding; protein binding; metal ion binding; unfolded protein binding; structural constituent of eye lens

Biological Process: visual perception; negative regulation of intracellular transport; response to stimulus; protein refolding; protein homooligomerization; negative regulation of apoptosis

Disease: Cataract 9, Multiple Types