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Flow Cytometric (FC) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant ALDH3A1 protein (red) or empty vector (blue) stained using ALDH3A1 antibody)

Mouse ALDH3A1 Monoclonal Antibody | anti-ALDH3A1 antibody

ALDH3A1 antibody

Gene Names
ALDH3A1; ALDH3; ALDHIII
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
ALDH3A1 antibody was purified by affinity chromatography.
Synonyms
ALDH3A1; Monoclonal Antibody; ALDH3A1 antibody; Monoclonal ALDH3A1; Anti-ALDH3A1; ALDHIII; ALDH3; Aldehyde dehydrogenase 3 family protein; AHD4; AHDC; ALDH III; Aldehyde dehydrogenase 3; Aldehyde dehydrogenase 3 family member A1; Aldehyde dehydrogenase 3A1; Aldehyde dehydrogenase class 3; Aldehyde dehydrogenase dimeric NADP preferring; Aldehyde dehydrogenase family 3 member A1; Aldehyde dehydrogenase family 3 subfamily A1; Aldehyde dehydrogenase isozyme 3; Aldehyde dehydrogenase type III; ALDH3A 1; MGC10406; Stomach aldehyde dehydrogenase; Tumor associated aldehyde dehydrogenase tumor ALDH; ALDHA1 3; ALDHA1-3; anti-ALDH3A1 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
1C6
Purity/Purification
ALDH3A1 antibody was purified by affinity chromatography.
Form/Format
Purified antibody supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.96 mg/ml (varies by lot)
Sequence Length
453
Applicable Applications for anti-ALDH3A1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
IHC: 1:50
WB: 1:2000
Biological Significance
ALDH3A1, also known as Aldehyde dehydrogenase 3 family member A1, is involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea.
Cross-Reactivity
Human
Immunogen
Full length human recombinant protein of human ALDH3A1 produced in HEK293T cell.
Preparation and Storage
Store at 4°C short term
Store at -20°C long term.

Flow Cytometric (FC)

(Flow Cytometric analysis of HEK293T cells transfected with either recombinant ALDH3A1 protein (red) or empty vector (blue) stained using ALDH3A1 antibody)

Flow Cytometric (FC) (Flow Cytometric analysis of HEK293T cells transfected with either recombinant ALDH3A1 protein (red) or empty vector (blue) stained using ALDH3A1 antibody)

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALDH3A1 protein (Right) or empty vector (Left) detected with ALDH3A1 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ALDH3A1 protein (Right) or empty vector (Left) detected with ALDH3A1 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of ALDH3A1 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using ALDH3A1 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of ALDH3A1 protein in paraffin embedded Adenocarcinoma of Human ovary tissue using ALDH3A1 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant ALDH3A1 protein using ALDH3A1 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant ALDH3A1 protein using ALDH3A1 antibody)
Related Product Information for anti-ALDH3A1 antibody
Mouse monoclonal ALDH3A1 antibody
Product Categories/Family for anti-ALDH3A1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
218
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
aldehyde dehydrogenase, dimeric NADP-preferring
NCBI Official Synonym Full Names
aldehyde dehydrogenase 3 family, member A1
NCBI Official Symbol
ALDH3A1
NCBI Official Synonym Symbols
ALDH3; ALDHIII
NCBI Protein Information
aldehyde dehydrogenase, dimeric NADP-preferring
UniProt Protein Name
Aldehyde dehydrogenase, dimeric NADP-preferring
Protein Family
UniProt Gene Name
ALDH3A1
UniProt Synonym Gene Names
ALDH3
UniProt Entry Name
AL3A1_HUMAN

NCBI Description

Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2008]

Uniprot Description

ALDH3A1: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein preferentially oxidizes aromatic aldehyde substrates. It may play a role in the oxidation of toxic aldehydes. Belongs to the aldehyde dehydrogenase family.

Protein type: Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450; Amino Acid Metabolism - tyrosine; EC 1.2.1.5; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - histidine

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: nucleoplasm; extracellular space; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; plasma membrane; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; aldehyde dehydrogenase [NAD(P)+] activity; 3-chloroallyl aldehyde dehydrogenase activity; alcohol dehydrogenase (NADP+) activity

Biological Process: response to drug; response to cAMP; response to glucocorticoid stimulus; positive regulation of cell proliferation; response to hypoxia; aldehyde metabolic process; response to nutrient; aging

Research Articles on ALDH3A1

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Product Notes

The ALDH3A1 aldh3a1 (Catalog #AAA533466) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ALDH3A1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). FC: 1:100 IF: 1:100 IHC: 1:50 WB: 1:2000. Researchers should empirically determine the suitability of the ALDH3A1 aldh3a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH3A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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