Microsomal Glutathione S Transferase 1 Recombinant Protein | MGST1 recombinant protein

Recombinant Microsomal Glutathione S Transferase 1 (MGST1)

Cat. Num. AAA2031183

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Microsomal Glutathione S Transferase 1; Recombinant Microsomal Glutathione S Transferase 1 (MGST1); MGST1 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.1 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-MDN EVLMAFTSYA TIILTKMMFM SSATAFQRIT NKVFANPEDC AGFGKGENAK KFVRTDEKVE RVRRAHLNDL ENIVPFLGIG LLYSLSGPDL STALMHFRIF VGARIYHTIA YLTPLPQPNR GLAFFVGYGVTLSMAYRLLR SRLYL
• Sequence Length: 155

• Applicable Applications for MGST1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 49.1kDa
• Accurate Molecular Mass (KD): 47kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and GST-tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Met8~Leu155
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 19705453
• NCBI GeneID: 171341
• NCBI Accession #: NP_599176.1
• NCBI GenBank Nucleotide #: NM_134349.3
• Molecular Weight: 17,472 Da
• NCBI Official Full Name: microsomal glutathione S-transferase 1
• NCBI Official Synonym Full Names: microsomal glutathione S-transferase 1
• NCBI Official Symbol: Mgst1
• NCBI Protein Information: microsomal glutathione S-transferase 1
• UniProt Protein Name: Microsomal glutathione S-transferase 1
• Protein Family: Microsomal glutathione S-transferase
• UniProt Gene Name: Mgst1
• UniProt Synonym Gene Names: Gst12; Microsomal GST-1
• UniProt Entry Name: MGST1_RAT

NCBI Description

enzyme that catalyzes the transfer of glutathione onto hydrophobic substrates [RGD, Feb 2006]

Uniprot Description

MGST1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Has a wide substrate specificity. Belongs to the MAPEG family.

Protein type: Transferase; Endoplasmic reticulum; Membrane protein, integral; Mitochondrial; Membrane protein, peripheral; Membrane protein, multi-pass; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 2.5.1.18

Cellular Component: peroxisomal membrane; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; apical part of cell; mitochondrial inner membrane; integral to membrane; nucleus

Molecular Function: identical protein binding; protein homodimerization activity; glutathione binding; glutathione transferase activity; glutathione peroxidase activity

Biological Process: response to drug; Leydig cell differentiation; glutathione metabolic process; response to lipopolysaccharide; response to organic nitrogen