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Western Blot (WB) (Western Blot analysis of ALDH1A1 on human liver tissue lysate using anti-ALDH1A1 antibody at 1/10,000 dilution.)

Mouse anti-Human, Rat ALDH1A1 Monoclonal Antibody | anti-ALDH1A1 antibody

ALDH1A1 Monoclonal Antibody

Gene Names
ALDH1A1; ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
ProG purified.
Synonyms
ALDH1A1; Monoclonal Antibody; ALDH1A1 Monoclonal Antibody; RALDH 1; RalDH1; ALDC; ALDH1; PUMB1; Acetaldehyde dehydrogenase 1; AHD2; AL1A1; Aldehyde dehydrogenase 1 family member A1; Aldehyde dehydrogenase 1 soluble; Aldehyde dehydrogenase 1A1; Aldehyde dehydrogenase; Aldehyde dehydrogenase cytosolic; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase liver cytosolic; ALDH 1; ALDH 1A1; ALDH class 1; ALDH; liver cytosolic; ALDH-E1; ALDH1 A1; ALDH11; ALHDII; cytosolic; epididymis luminal protein 12; epididymis luminal protein 9; epididymis secretory sperm binding protein Li 53e; HEL-S-53e; MGC2318; Retinal dehydrogenase 1; anti-ALDH1A1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Specificity
ALDH1A1 monoclonal antibody detects endogenous levels of ALDH1A1 protein.
Purity/Purification
ProG purified.
Form/Format
Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
501
Applicable Applications for anti-ALDH1A1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:2,000-1:10,000
IHC: 1:50-1:200
Immunogen
Recombinant protein within human ALDH1A1 aa 5-200.
Preparation and Storage
Store at 4 degree C after thawing.
Aliquot store at -20 degree C.
Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of ALDH1A1 on human liver tissue lysate using anti-ALDH1A1 antibody at 1/10,000 dilution.)

Western Blot (WB) (Western Blot analysis of ALDH1A1 on human liver tissue lysate using anti-ALDH1A1 antibody at 1/10,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-ALDH1A1 antibody. Counter stained with hematoxylin.)
Related Product Information for anti-ALDH1A1 antibody
The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
216
UniProt Accession #
Molecular Weight
55kDa
NCBI Official Full Name
Retinal dehydrogenase 1
NCBI Official Synonym Full Names
aldehyde dehydrogenase 1 family member A1
NCBI Official Symbol
ALDH1A1
NCBI Official Synonym Symbols
ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e
NCBI Protein Information
retinal dehydrogenase 1
UniProt Protein Name
Retinal dehydrogenase 1
Protein Family
UniProt Gene Name
ALDH1A1
UniProt Synonym Gene Names
ALDC; ALDH1; PUMB1; RALDH 1; RalDH1
UniProt Entry Name
AL1A1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet. [provided by RefSeq, Mar 2011]

Uniprot Description

ALDH1A1: Binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid. Belongs to the aldehyde dehydrogenase family.

Protein type: GAPs, Ras; EC 1.2.1.36; GAPs; Cofactor and Vitamin Metabolism - retinol; Apoptosis; Oxidoreductase

Chromosomal Location of Human Ortholog: 9q21.13

Cellular Component: cytoplasm; cytosol

Molecular Function: aldehyde dehydrogenase (NAD) activity; androgen binding; 3-chloroallyl aldehyde dehydrogenase activity; benzaldehyde dehydrogenase (NAD+) activity; retinal dehydrogenase activity

Biological Process: retinol metabolic process; xenobiotic metabolic process; aldehyde metabolic process; ethanol oxidation

Research Articles on ALDH1A1

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Product Notes

The ALDH1A1 aldh1a1 (Catalog #AAA3014431) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ALDH1A1 Monoclonal Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ALDH1A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:2,000-1:10,000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the ALDH1A1 aldh1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALDH1A1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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