Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyroglutamylated RFamide peptide receptor (QRFPR) Standard Curve.)
Mouse Pyroglutamylated RFamide Peptide Receptor (QRFPR) ELISA Kit | QRFPR elisa kit
Mouse Pyroglutamylated RFamide Peptide Receptor (QRFPR) ELISA Kit
Gene Names
Qrfpr; AQ27; Gpr103; SP9155
Reactivity
Mouse
Synonyms
Pyroglutamylated RFamide Peptide Receptor (QRFPR); Mouse Pyroglutamylated RFamide Peptide Receptor (QRFPR) ELISA Kit; QRFPR; AQ27; GPR103; MGC149217; SP9155; G protein-coupled receptor 103; OTTHUMP00000164030; QRFP receptor; QRFPR elisa kit
Reactivity
Mouse
Specificity
Mouse Pyroglutamylated RFamide peptide receptor (QRFPR) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse QRFPR. No significant cross-reactivity or interference between Mouse QRFPR and analogues was observed.
Sequence Length
433
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyroglutamylated RFamide peptide receptor (QRFPR) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Mouse Pyroglutamylated RFamide peptide receptor (QRFPR) Standard Curve.)
Related Product Information for QRFPR elisa kit
Description: This Mouse Pyroglutamylated RFamide peptide receptor (QRFPR) ELISA Kit employs a two-site sandwich ELISA to quantitate QRFPR in samples. An antibody specific for QRFPR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any QRFPR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QRFPR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QRFPR bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: GPR103 shares between 35% and 38% sequence identity in the transmembrane regions with various peptide receptors, including neuropeptide FF2, neuropeptide Y2, and galanin GalR1 receptors. Northern blot analysis of human brain regions revealed widespread expression, including transcripts of 4.0, 2.6 and 1.4 kb in the thalamus and hypothalamus, with a further 9.5-kb transcript in the hypothalamus and a 1.4-kb transcript in the pituitary.The deduced 431-amino acid protein contains the 7-transmembrane structure typical of G protein-coupled receptors. GPR103 shares 49% identity with NPFF1 (GPR147) and NPFF2 (GPR74). Quantitative PCR analysis detected highest GPR103 expression in brain, predominantly in retina, trigeminal ganglion, hypothalamus, and vestibular nucleus
Background: GPR103 shares between 35% and 38% sequence identity in the transmembrane regions with various peptide receptors, including neuropeptide FF2, neuropeptide Y2, and galanin GalR1 receptors. Northern blot analysis of human brain regions revealed widespread expression, including transcripts of 4.0, 2.6 and 1.4 kb in the thalamus and hypothalamus, with a further 9.5-kb transcript in the hypothalamus and a 1.4-kb transcript in the pituitary.The deduced 431-amino acid protein contains the 7-transmembrane structure typical of G protein-coupled receptors. GPR103 shares 49% identity with NPFF1 (GPR147) and NPFF2 (GPR74). Quantitative PCR analysis detected highest GPR103 expression in brain, predominantly in retina, trigeminal ganglion, hypothalamus, and vestibular nucleus
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pyroglutamylated RF-amide peptide receptor
NCBI Official Synonym Full Names
pyroglutamylated RFamide peptide receptor
NCBI Official Symbol
Qrfpr
NCBI Official Synonym Symbols
AQ27; Gpr103; SP9155
NCBI Protein Information
pyroglutamylated RF-amide peptide receptor
UniProt Protein Name
Pyroglutamylated RFamide peptide receptor
Protein Family
UniProt Gene Name
Qrfpr
UniProt Synonym Gene Names
Gpr103
UniProt Entry Name
QRFPR_MOUSE
Research Articles on QRFPR
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Product Notes
The Mouse QRFPR qrfpr (Catalog #AAA9717190) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9717190 ELISA Kit recognizes Mouse QRFPR. It is sometimes possible for the material contained within the vial of "Pyroglutamylated RFamide Peptide Receptor (QRFPR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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