Mouse Akt, pan Monoclonal Antibody | anti-Akt1 antibody

Akt, pan (Rac PKa, PKBa) (Biotin)

Cat. Num. AAA605411

• Gene Names: Akt1; akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Ascites; Ascites
• Synonyms: Akt; pan; Monoclonal Antibody; pan (Rac PKa; PKBa) (Biotin); Anti -Akt; anti-Akt1 antibody

• Host: Mouse
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: 10g142
• Specificity: Recognizes endogenous levels of total Akt protein. Does not crossreact with other related proteins. Species crossreactivity: Mouse, rat and monkey.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in 136mM sodium chloride, 2.6mM KCl, 12mM sodium phosphate dibasic, pH 7.4, 2mg/ml BSA, and 50% glycerol.

• Applicable Applications for anti-Akt1 antibody: Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, Western Blot and Immunoprecipitation.; Dilution: Flow Cytometry: 1:50; Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide corresponding to residues in the carboxy-terminal sequence of human Akt protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from various cell lines using Akt (pan) (40D4) Mouse mAb (Biotinylated).)

Western Blot (WB)

(Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 (1) or Calyculin A (2) using Akt (pan) (40D4) Mouse mAb (Biotinylated) and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate). Western blot analysis was performed using Akt (pan) (C67E7) Rabbit mAb.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells using Akt (pan) (40D4) Mouse mAb (Biotinylated) (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) (red).)

Related Product Information for anti-Akt1 antibody

Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis (1-3). This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase (2,3). Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 (4) and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2 responsible for phosphorylation of Akt at Ser473 has been identified as mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with rictor and Sin1 (5,6). Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including Bad (7), forkhead transcription factors (8), c-Raf (9) and caspase-9. PTEN phosphatase is a major negative regulator of the PI3 kinase/Akt signaling pathway (10). LY294002 is a specific PI3 kinase inhibitor (11). Another essential Akt function is the regulation of glycogen synthesis through phosphorylation and inactivation of GSK-3alpha and beta (12,13). Akt may also play a role in insulin stimulation of glucose transport (12). In addition to its role in survival and glycogen synthesis, Akt is involved in cell cycle regulation by preventing GSK-3beta mediated phosphorylation and degradation of cyclin D1 (14) and by negatively regulating the cyclin dependent kinase inhibitors p27 Kip (15) and p21 Waf1/CIP1 (16). Akt also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor (17). More importantly, Akt phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex (18). Inhibition of mTOR stops the protein synthesis machinery by inactivating p70 S6 kinase and activating the eukaryotic initiation factor 4E binding protein 1 (4E-BP1), an inhibitor of translation (18,19).

Product Categories/Family for anti-Akt1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 24647360
• NCBI GeneID: 41957
• NCBI Accession #: NP_732115.1
• NCBI GenBank Nucleotide #: NM_169707.1
• UniProt Accession #: Q8INB9; Q0KI65; Q24293; Q24469; Q24470; Q7JN11; Q8T9A5; Q9VEY7
• Molecular Weight: 68,485 Da
• NCBI Official Full Name: Akt1, isoform B
• NCBI Official Symbol: Akt1
• NCBI Official Synonym Symbols: akt; Akt; AKT; Akt/PKB; AKT/PKB; akt1; AKT1; Akt1|PKB; CG4006; D-Akt; dakt; dAkt; dAKT; Dakt; DAkt; dAKT/dPKB; dAkt/PKB; dakt1; dAkt1; dAKT1; Dakt1; DAkt1; DAKT1; DAKT1/PKB; DmelCG4006; dPKB; Dpkb; DPKB; DRAC-PK; DRAC-PK66; DRAC-PK85; l(3)04226; l(3)89Bq;
• NCBI Protein Information: CG4006 gene product from transcript CG4006-RA; Akt kinase; Akt1; Akt1-PA; Akt1-PB; Akt1-PC; CG4006-PA; CG4006-PB; CG4006-PC; dAkt kinase; protein kinase B; related to PKA to PKC protein kinases; related to the A and C kinases
• UniProt Protein Name: RAC serine/threonine-protein kinase
• Protein Family: Potassium channel
• UniProt Gene Name: Akt1
• UniProt Synonym Gene Names: DAkt; DRAC-PK; Dakt1; PKB
• UniProt Entry Name: AKT1_DROME

Uniprot Description

Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.

Protein type: Kinase, protein; Protein kinase, AGC; Oncoprotein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); AGC group; AKT family

Cellular Component: cell surface; cell soma; cytoplasm; plasma membrane

Molecular Function: protein serine/threonine kinase activity; phosphoinositide binding; ATP binding; protein kinase activity

Biological Process: oogenesis; circadian rhythm; positive regulation of cell size; chitin-based embryonic cuticle biosynthetic process; regulation of dendrite development; positive regulation of multicellular organism growth; protein amino acid phosphorylation; somatic muscle development; regulation of protein import into nucleus; regulation of cell shape; sequestering of lipid; myoblast fusion; negative regulation of synaptic growth at neuromuscular junction; positive regulation of axon regeneration; multicellular organism growth; regulation of multicellular organism growth; regulation of organ growth; positive regulation of organ growth; positive regulation of cell growth; epithelial cell migration, open tracheal system; dendrite regeneration; regulation of hemocyte proliferation; open tracheal system development; insulin receptor signaling pathway; response to oxidative stress; lipid metabolic process; regulation of cell size; negative regulation of apoptosis