Mouse anti-Human, Mouse Akt, dephosphorylated Monoclonal Antibody
Akt, dephosphorylated (Ser473) (Rac PKa, PKBa)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
PurifiedPurified.
Synonyms
Akt; dephosphorylated; Monoclonal Antibody; dephosphorylated (Ser473) (Rac PKa; PKBa); Anti -Akt; anti-Akt; dephosphorylated antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
6D108
Specificity
Recognizes human Akt dephosphorylated at Ser473. Does not cross-react with Akt phosphorylated at Ser473. Species Crossreactivity: mouse.
Purity/Purification
Purified
Purified.
Purified.
Form/Format
Supplied as a lyophilized powder in PBS, pH 7.2, 0.1% sodium azide PEG and sucrose. Reconstitute with 1ml sterile, ddH2O. (15 min, RT).
Applicable Applications for anti-Akt, dephosphorylated antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.05ug/ml
Western Blot: 1ug/ml (AP)
Western Blot: 0.5 ug/ml (HRP/ECL)
Dilution: ELISA: 0.05ug/ml
Western Blot: 1ug/ml (AP)
Western Blot: 0.5 ug/ml (HRP/ECL)
Immunogen
Synthetic peptide corresponding to C-terminal (QFSYSA) human Akt (KLH).
Positive Control
A1124-02D1 Cell lysate from untreated HepG2 cells.
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Related Product Information for anti-Akt, dephosphorylated antibody
The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kiinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far; the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.
Product Categories/Family for anti-Akt, dephosphorylated antibody
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Product Notes
The Akt, dephosphorylated (Catalog #AAA602934) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Akt, dephosphorylated (Ser473) (Rac PKa, PKBa) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Akt, dephosphorylated can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.05ug/ml Western Blot: 1ug/ml (AP) Western Blot: 0.5 ug/ml (HRP/ECL). Researchers should empirically determine the suitability of the Akt, dephosphorylated for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Akt, dephosphorylated, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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