Testing Data
(EGF-treated A431 cells were fixed, permeabilized, and stained with MBS609322 (2ug/ml) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor 488 (green) was used to stain actin.)
Mouse AKT Monoclonal Antibody | anti-AKT antibody
AKT, phosphorylated (Ser473) (Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1) (PE)
Testing Data
(EGF-treated A431 cells were fixed, permeabilized, and stained with MBS609322 (2ug/ml) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor 488 (green) was used to stain actin.)
Testing Data
(EGF-treated A431 cells were fixed, permeabilized, and stained with MBS609322 (2ug/ml) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor 488 (green) was used to stain actin.)
Testing Data
(A431 cells were either untreated (left) or treated with EGF (right). Cells were fixed, permeabilized and stained with MBS609322 (2ug/ml) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor488 (green) was used to stain actin.)
Testing Data
(A431 cells were either untreated (left) or treated with EGF (right). Cells were fixed, permeabilized and stained with MBS609322 (2ug/ml) (red). Cy3-conjugated anti-mouse was used for detection and Phalloidin-AlexaFluor488 (green) was used to stain actin.)
Immunohistochemistry (IHC)
(Representative staining pattern/morphology on a breast cancer array spot. Pretreatment with citrate pH 6, antigen retrieval. MBS609322 was diluted to 1:200, IHC-Select Detection with HRP-DAB. Imunoreactivity is seen as strong cytoplasmic staining of ductal carcinoma cells (brown color).)
Immunohistochemistry (IHC)
(Representative staining pattern/morphology on a breast cancer array spot. Pretreatment with citrate pH 6, antigen retrieval. MBS609322 was diluted to 1:200, IHC-Select Detection with HRP-DAB. Imunoreactivity is seen as strong cytoplasmic staining of ductal carcinoma cells (brown color).)
Testing Data
(Representative staining pattern/morphology of malignant melanoma array spot. Pretreatment with citrate pH 6, antigen retrieval.)
Testing Data
(Representative staining pattern/morphology of malignant melanoma array spot. Pretreatment with citrate pH 6, antigen retrieval.)
SDS-Page
(PDGF-treated treated (lane 1) and untreated (lane 2) NIH/3T3 cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed MBS609322 (0.5ug/ml).)
SDS-Page
(PDGF-treated treated (lane 1) and untreated (lane 2) NIH/3T3 cell lysate was resolved by SDS-PAGE, transferred to PVDF, and probed MBS609322 (0.5ug/ml).)
NCBI and Uniprot Product Information
Uniprot Description
Akt1: an oncogenic AGC kinase that plays a critical role in regulating cell survival and metabolism in many different signaling pathways. Dual phosphorylation is required for its activation. T308 is phosphorylated by PDK1 in the PI3 kinase pathway, and S473 is phosphorylated by mTOR in the mTORC2 pathway. The 'Lys-63'-linked ubiquitination of AKT1 by TRAF6 is important for its translocation to the plasma membrane, phosphorylation, and activation. When Akt is fully phosphorylated it translocates into the nucleus, undergoes 'Lys-48'-polyubiquitination catalyzed by TTC3, leading to its proteosomal degradation. Hyperactive or overexpressed in a number of cancers including breast, prostate, lung, pancreatic, liver, ovarian and colorectal. Over 160 protein substrates are known including many that regulate transcription, metabolism, apoptosis, cell cycle, and growth.
Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Oncoprotein; Kinase, protein; AGC group; AKT family
Chromosomal Location of Human Ortholog: 14q32.32
Cellular Component: nucleoplasm; microtubule cytoskeleton; mitochondrion; cytoplasm; plasma membrane; spindle; intercellular junction; nucleus; cytosol
Molecular Function: identical protein binding; protein serine/threonine kinase activity; protein binding; phosphatidylinositol-3,4,5-triphosphate binding; enzyme binding; protein kinase C binding; nitric-oxide synthase regulator activity; protein serine/threonine/tyrosine kinase activity; kinase activity; phosphatidylinositol-3,4-bisphosphate binding; ATP binding; protein kinase activity
Biological Process: negative regulation of JNK cascade; positive regulation of nitric oxide biosynthetic process; regulation of myelination; nerve growth factor receptor signaling pathway; protein ubiquitination; glucose homeostasis; regulation of cell migration; protein amino acid phosphorylation; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; germ cell development; positive regulation of glucose import; cell projection organization and biogenesis; protein catabolic process; maternal placenta development; response to food; platelet activation; glycogen biosynthetic process; fibroblast growth factor receptor signaling pathway; positive regulation of nitric-oxide synthase activity; positive regulation of blood vessel endothelial cell migration; glucose metabolic process; positive regulation of lipid biosynthetic process; positive regulation of cell growth; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; response to heat; T cell costimulation; positive regulation of fat cell differentiation; negative regulation of protein kinase activity; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; positive regulation of transcription factor activity; response to oxidative stress; regulation of nitric-oxide synthase activity; negative regulation of apoptosis; negative regulation of autophagy; negative regulation of fatty acid beta-oxidation; translation; apoptosis; protein amino acid autophosphorylation; regulation of glycogen biosynthetic process; positive regulation of cellular protein metabolic process; positive regulation of glycogen biosynthetic process; negative regulation of cell size; negative regulation of caspase activity; glucose transport; signal transduction; nitric oxide metabolic process; regulation of translation; apoptotic mitochondrial changes; protein kinase B signaling cascade; inflammatory response; nitric oxide biosynthetic process; cell differentiation; activated T cell apoptosis; aging; negative regulation of proteolysis; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; myelin maintenance in the peripheral nervous system; protein modification process; endocrine pancreas development; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; cell proliferation; G-protein coupled receptor protein signaling pathway; peptidyl-serine phosphorylation; protein import into nucleus, translocation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; insulin receptor signaling pathway; positive regulation of vasoconstriction; innate immune response; gene expression; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; hyaluronan metabolic process
Disease: Schizophrenia; Cowden Syndrome 6; Proteus Syndrome; Breast Cancer; Ovarian Cancer
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Product Notes
The AKT akt1 (Catalog #AAA6244599) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AKT, phosphorylated (Ser473) (Protein kinase B, RAC-alpha serine/threonine-protein kinase, murine thymoma viral (v-akt) oncogene homolog-1, rac protein kinase alpha, v-akt murine thymoma viral oncogene homolog 1) (PE) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKT can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the AKT akt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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