Rabbit anti-Human AKR1B1  Monoclonal Antibody | anti-AKR1B1  antibody

Rabbit anti Human AKR1B1  Monoclonal Antibody

Cat. Num. AAA2544224

• Gene Names: AKR1B1; AR; ADR; ALR2; ALDR1
• Reactivity: Human
• Applications: ELISA
• Synonyms: AKR1B1 ; Monoclonal Antibody; Rabbit anti Human AKR1B1 Monoclonal Antibody; anti-AKR1B1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Sequence Length: 172

• Applicable Applications for anti-AKR1B1 antibody: ELISA (EIA)
• Application Notes: ELISA: 0.1-0.2 mug/mL. This antibody can be used at 0.1-0.2 mug/mL with the appropriate secondary reagents to detect Human AKR1B1. The detection limit for Human AKR1B1 is approximately 0.0049 ng/well.
• Immunogen: Recombinant Human AKR1B1 protein
• Buffer: 0.2 mum filtered solution in PBS with 5% trehalose
• Preparation and Storage: This antibody can be stored at 2 degree C- 8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-AKR1B1  antibody

Aldose reductase (AKR1B1) belongs to the aldo/keto reductase superfamily. AKR1B1 is a NADPH-dependent aldo-keto reductase best known as the rate-limiting enzyme of the polyol pathway. Expression of AKR1B1 was the highest in lens and retina. It is the first enzyme in the polyol pathway through which glucose is converted to sorbitol which is important for the function of various organs in the body, and has been implicated in the etiology of diabetic complications. AKR1B1 is quite abundant in the collecting tubule cells and thought to provide protection against hypertonic environment. Some human tissues contain AKR1B1 as well as AKR1B10, a closely related member of the aldo-keto reductase superfamily. 

NCBI and Uniprot Product Information

• NCBI GI #: 1070575232
• NCBI GeneID: 231
• NCBI GenBank Nucleotide #: NM_001346142.1
• Molecular Weight: 35,853 Da
• NCBI Official Full Name: aldose reductase isoform 2
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member B
• NCBI Official Symbol: AKR1B1
• NCBI Official Synonym Symbols: AR; ADR; ALR2; ALDR1
• NCBI Protein Information: aldose reductase
• UniProt Protein Name: Aldose reductase
• UniProt Gene Name: AKR1B1
• UniProt Synonym Gene Names: ALDR1; AR
• UniProt Entry Name: ALDR_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

Uniprot Description

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - pyruvate; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q35

Cellular Component: cytoplasm; cytosol; nucleoplasm

Molecular Function: aldehyde reductase activity; aldo-keto reductase activity; electron carrier activity; glyceraldehyde oxidoreductase activity

Biological Process: C21-steroid hormone biosynthetic process; carbohydrate metabolic process; fructose biosynthetic process; response to stress