Immunoprecipitation (IP)
(AKR1B1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate2 uL anti-AKR1B1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AKR1B1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)
Rabbit anti-Human AKR1B1 Polyclonal Antibody | anti-AKR1B1 antibody
Anti-AKR1B1 Antibody, Rabbit Polyclonal
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunoprecipitation (IP)
(AKR1B1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate2 uL anti-AKR1B1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AKR1B1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)
Immunoprecipitation (IP)
(AKR1B1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg A431 Whole Cell Lysate2 uL anti-AKR1B1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-AKR1B1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size: 35 kDa)
Western Blot (WB)
(Anti-AKR1B1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF-7 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-AKR1B1 rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLane B: MCF-7 Whole Cell LysateLane C: Jurkat Whole Cell LysateLane D: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:36 kDaObserved band size:34 kDa(We are unsure as to the identity of these extra bands.))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
Uniprot Description
AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.
Protein type: Carbohydrate Metabolism - pentose and glucuronate interconversions; Oxidoreductase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - galactose; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - fructose and mannose
Chromosomal Location of Human Ortholog: 7q35
Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol
Molecular Function: glyceraldehyde oxidoreductase activity; electron carrier activity; aldo-keto reductase activity; aldehyde reductase activity
Biological Process: steroid metabolic process; carbohydrate metabolic process; C21-steroid hormone biosynthetic process; response to stress
Research Articles on AKR1B1
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Product Notes
The AKR1B1 akr1b1 (Catalog #AAA8105241) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-AKR1B1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AKR1B1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the AKR1B1 akr1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKR1B1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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